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- 积分
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《Bovine CD49 positive-cell subpopulation remarkably increases in2 @6 S6 t, h3 F6 s7 _
mammary epithelial cells that retain a stem-like phenotype》---Research in Veterinary Science( y5 C" R" C; m$ J* Y7 J, V
We previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential
6 E, h: Q6 V# T+ ^2 N9 H9 M, e' |9 sto generate a functional mammary outgrowth. The aim of this study was to investigate on the) Q, E( l* R+ e3 l Z
immunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry
& j- W/ y F8 r9 `8 d& nanalysis showed different subpopulations of mammary epithelial cells emerging according to the timing of, T2 Z8 u3 v1 ?5 [! Q
cell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed,) c: i! j4 `2 A) f" x) [6 p1 ?
even if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed
1 \. p6 u5 W8 ufor CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative
5 K6 E+ x: ]- n% ccapabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when x4 F/ N4 C8 F0 O& A
transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation
; G! B0 z' Q" S9 ethat retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion" }5 \7 S1 Q7 i) g0 ^& j( u0 t- y
of the primitive population of adult mammary stem cells. |
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