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《Bovine CD49 positive-cell subpopulation remarkably increases in& m6 K% |7 u/ s/ i7 p3 I3 }5 X
mammary epithelial cells that retain a stem-like phenotype》---Research in Veterinary Science
! m& }& M5 J$ RWe previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential
0 p# L- O Q# W6 C& F, _, z6 F# L& jto generate a functional mammary outgrowth. The aim of this study was to investigate on the
# G$ r7 b+ E- ?! ^9 Gimmunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry
- K) o6 R" L" ~8 `0 kanalysis showed different subpopulations of mammary epithelial cells emerging according to the timing of
" w1 `* c2 S. B+ K3 [- z! {cell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed,% i6 t6 M( ~+ @/ @& x
even if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed
$ H: E9 S5 u: Z- J. n" afor CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative
6 q* f. m/ w. V1 B" Y* l/ kcapabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when3 b. I8 A0 e3 A$ @
transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation/ J6 ]! {& n, u. a
that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion# \3 G5 ]. c; e1 L; S& ]
of the primitive population of adult mammary stem cells. |
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