PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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PDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press$ f% \% w1 C6 H* p. U  p
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主要内容如下：
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Contents
6 e/ b, N1 @; L7 F6 APreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii1 b( h& r0 s! d3 Q7 c
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi, D2 p+ p& w# \$ {& R9 }
  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1$ M' Y7 G/ ^3 t- g- F
Christina Philippeos, Robin D. Hughes, Anil Dhawan, 2 F" [  e7 P1 Z$ R
and Ragai R. Mitry" }8 ~" m2 W7 z( L
  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate , F1 w* y8 M) u4 p7 S4 \
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  156 a2 o8 e; s3 D, Z* R9 Q' D4 Q
Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
1 z3 u% J: V+ i6 u9 ~4 K0 F  3  Isolation, Cultivation, and Application of Human Alveolar
/ q& v5 y  M9 o" yEpithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
3 D# o' i" G5 f7 N* R9 U8 BNicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, 3 R% d5 W5 G4 ?2 }; q- O  _
Hanno Huwer, and Claus-Michael Lehr7 y) K% k- m  q2 d
  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  436 D; B; B9 ?( D9 P9 N: T
Peyman Björklund and Per Hellman  k$ N) s, H5 k( j
  5  Functional Analysis of Human Islets of Langerhans Maintained
2 \: e: q# \1 e- l- }/ }in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55) ]- D' q/ \  G
Shanta J. Persaud, Bo Liu, and Peter M. Jones
' R2 p4 w' T5 `' j- ]% f1 ?- L  6  Conversion of Non-endocrine Human Pancreatic Cells / }* F& U. \) U
to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73
& ~( @, E6 F0 o3 a" C! a4 ?Min Zhao and Guo Cai Huang' y! x9 G8 P* R9 r  ~; n
  7  Evaluation of Cytochrome P450 Activities in Human ) s2 \$ D6 {8 n0 O* _
Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  87
$ P; c2 Y4 m* w! P: ]9 vMaría José Gómez-Lechón, Agustín Lahoz, José V. Castell,
: \. R: M2 F; H5 V! t0 O% Dand María Teresa Donato. w! q' |7 V0 a! B" q" d& W9 j
  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99- C6 F$ ^; E0 H) q
Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka,
& G  }: f9 I4 x+ I7 ]' K+ JPeter Büchler, Michael Schoenberg, Jörg Kleeff,
! c0 U$ a- Z& N; t, G- J3 QWolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy,
$ d; C4 I9 F' n( m, r. l# T  K6 w( wJan Hengstler, and Andreas K. Nussler
5 _3 f/ R2 o( C2 i, ?  9  Preclinical Testing of Virotherapeutics for Primary and Secondary ; @. W  Y4 F' q, j/ T( T; H
Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121  r" B8 Z; |- R5 N
Martina Zimmermann, Timo Weiland, Michael Bitzer,
6 F2 i0 G+ p/ y* I9 V; [and Ulrich M. Lauer
- p3 g7 W* e  F, \( l) S5 z10  Isolation and Functional Studies of Human Fetal Gastric . |" i& Z, e. l3 a0 s  _
Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137* L, x9 @9 [2 Y: h3 H
Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard' S! x4 `! h" T$ x, y
11  Isolation, Characterization, and Culture of Normal Human & M# m* P9 P% Y, J+ M/ a0 B
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157: H" L+ e: {. C/ U* K$ q: T
Jean-François Beaulieu and Daniel Ménard
+ B( q) K4 C5 r" c& gx Contents
. x3 _' f& g4 c! d12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells
( A1 v6 L  p0 D$ i4 nand Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175
" o0 y" R, D, K& n4 wClaire C. Sharpe and Mark E.C. Dockrell, ~2 ?9 l6 N; C) Q1 m4 o
13  Glomerular Epithelial and Mesangial Cell Culture . C2 C- B9 Z; t
and Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187
& V2 ^5 e! Y1 GHeather M. Wilson and Keith N. Stewart
4 h  x& ~& x; }9 R/ B; z0 A; C14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203, x! m, L  r& T* @  |/ W1 |
Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried
4 @" q2 n( v8 ]8 f, P; l15  Primary Culture of Human Adipocyte Precursor Cells:
" B9 ?6 J& X: V$ y  HExpansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215. N" m2 Y! j4 s; k/ e2 G- h8 s
Thomas Skurk and Hans Hauner
: ^8 O  A% o3 {: k+ L4 b9 e- a3 W" H16  Primary Culture of Ovarian Cells for Research on Cell Interactions
& R, _$ F$ n* ein the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227
* q* {+ e( `( g# k( ~Jerzy F. Galas
3 A4 Q3 K8 O% J3 s- h% B0 ]9 s1 {' V! c17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251
; |& @& M! {8 r' e4 O1 jDiane Proudfoot and Catherine Shanahan* y2 O: b% H$ ]/ c$ X  k
18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265
$ @9 {5 k1 @" Y; z8 D/ @% H; bRichard C.M. Siow2 @7 L( t  A% L- L' s) K+ F/ r
19  Human Peripheral Blood Mononuclear Cell Culture for Flow
& q( [( j# I* e. XCytometric Analysis of Phosphorylated Mitogen-Activated ) d/ b+ m7 b8 ~: ?1 g2 a
Protein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275
9 |1 n8 i- X5 QAthanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou,
5 m+ \5 x+ o3 o& V8 ~: [0 K9 Pand Dimitrios P. Bogdanos7 d0 q, g( d+ [
20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287
( l2 N- S/ p. A  wHaibin Su, Maria Serena Longhi, Pengyun Wang,
) C  Y5 `! r* I( \# U* HDiego Vergani, and Yun Ma' i, U/ f/ q2 L  x5 h
21  Human Chondrocyte Cultures as Models of Cartilage-Specific * B# I8 l- \- B( i  |- g
Gene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301
- J8 K& ^& C' n" }. \+ uMiguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, ( g- T- n7 l$ F; J" Y
Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring& S. E8 P0 p1 `
22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337
( i9 j" F9 G+ jAlison Gartland, Robin M.H. Rumney, Jane P. Dillon,
# Z& d! ^% e, I: W5 Oand James A. Gallagher
. l, L! a' N2 Y23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357
+ Z3 D3 h  s9 J# K/ B, m  v: l* B8 RAnkita Agrawal, James A. Gallagher, and Alison Gartland! {0 O2 ]  r( L/ v5 |
24  Effects of Temperature Generated from the Holmium: YAG Laser # i1 P8 Y2 B* X# K/ C$ y
on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377
/ t/ c0 n( V. H# Z$ N  kMoustafa I. Hafez, Anne Sandison, Richard R.H. Coombs,
! L# Q7 \2 V1 a) kIan D. McCarthy, and Al-Shymaa M. Hafez
9 _9 l# A+ D6 t( l, G3 I: F25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  3850 B" E/ z  W% L9 k
Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia
( W2 A. c% t# h1 |; d3 u  j+ t$ H26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  3939 A4 r/ c) e! f  c
Robert Thomas and Elizabeth Ratcliffe* Y8 g5 A) t8 s- e3 _. d
27  Culturing and Differentiating Human Mesenchymal Stem Cells 1 }  K. y$ i2 t& l: K" |9 T' z
for Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  407
3 ]0 [7 {7 t7 O4 V0 [( a2 nWilliam R. Otto and Catherine E. Sarraf
% q! l. z0 E5 i* e) C& K/ ]! YIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  4270 T4 `' C6 m' }' D4 e9 D

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