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亮亮燕燕

Three-dimensional type I collagen gel system for the study of osteoblastic metastases produced by metastatic prostate cancer.
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! n2 s9 ^1 R. e2 x: r$ u5 g7 J$ lKoutsilieris M1, Sourla A, Pelletier G, Doillon CJ.
) M" Z. O" _! W  l4 K7 o' mAuthor information
+ I. C: p: F% T3 x* ?6 HAbstract
  p( l9 Y; x& H$ J( ?! I+ Q! m8 {We developed a three-dimensional type I collagen gel cell culture system that allows coculturing of human MG-63 osteoblast-like cells and various human cancer cells. Inoculation of human PC-3 metastatic prostate cancer cells into this type I collagen gel containing human MG-63 osteoblast-like cells produced an osteoblastic-like reaction that presented as an increased number of MG-63 cells and increased density of type I collagen around MG-63 cells adjacent to inoculated PC-3 cells by microscope analysis. Under identical experimental conditions, inoculation of cell-free medium, human KLE endometrial adenocarcinoma cells, and Calu-1 lung cancer cells did not produce this blastic-like reaction. In situ hybridization documented the uniform expression of insulin-like growth factor I (IGF-I) and of urokinase-type plasminogen activator (uPA) mRNA in MG-63 and PC-3 cells separately cultured in this substrata. The uniform expression of uPA was also documented by immunocytochemistry using a monoclonal and a polyclonal antihuman uPA antibody. The relative expression of uPA was higher in PC-3 cells than in MG-63, KLE, and Calu-1 cancer cells. We conclude that this novel cell culture system may become a useful model to study the pathophysiology of the osteoblastic reaction in vitro.8 q9 r- B6 r+ S8 i# l: A
PMID: 7863832 DOI: 10.1002/jbmr.5650091120

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