- 积分
- 256
- 威望
- 256
- 包包
- 378
|
本帖最后由 xyzengh 于 2009-9-8 22:39 编辑 + X9 y6 [( \) {- T. E5 `2 v( A
2 ]. x- z6 Q& R, W. x sOct4是核抗原,所以triton是必须的,SSEA3/4是膜表面抗原,可加可不加,你为了方便可以都加,把我文章里面的方法发上来给你参考吧。( O4 v2 |1 z0 u- Q, A/ V
, h2 a+ l. J( k3 X. u
Immunofluorescence Analysis
# b* @+ k0 U' A8 N8 o6 S5 K" DCells were fixed with 4% paraformaldehyde for 10 min., incubated in PBS containing 5% rabbit serum and 0.4% Triton X-100 for blocking and permeablization, respectively. PBS containing 0.5% Tween 20 (PBS-T) was used to dilute antibodies and wash the cells in the following procedures. The cells were incubated with antibodies against OCT4, SSEA-4,TRA-1-60 and TRA-1-81 at 37 °C for 1 h, followed by washing with PBS-T for three times. Afterwards, the cells were incubated with fluorochrome-conjugated, corresponding secondary antibodies at 37 °C for 45 min. and washed with PBS-T for three times. Finally, the cells were examined under fluorescence microscope to capture both phase and fluorescent images. |
-
总评分: 威望 + 10
包包 + 10
查看全部评分
|