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Centrifugal seeding of mammalian cells in nonwoven fibrous matrices.
. G u" q1 q% oNg R, Gurm JS, Yang ST.
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, `+ _% S: z. H% X5 uWilliam G. Lowrie Dept. of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA.
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Three-dimensional (3D) cell cultures have many advantages over two-dimensional cultures. However, seeding cells in 3D scaffolds such as nonwoven fibrous polyethylene terephthalate (PET) matrices has been a challenge task in tissue engineering and cell culture bioprocessing. In this study, a centrifugal seeding method was investigated to improve the cell seeding efficiency in PET matrices with two different porosities (93% and 88%). Both the centrifugal force and centrifugation time were found to affect the seeding efficiency. With an appropriate centrifugation speed, a high 80-90% cell seeding efficiency was achieved and the time to reach this high seeding efficiency was less than 5 min. The seeding efficiency was similar for matrices with different porosities, although the optimal seeding time was significantly shorter for the low-porosity scaffold. Post seeding cell viability was demonstrated by culturing colon cancer cells seeded in PET matrices for over 5 days. The centrifugal seeding method developed in this work can be used to efficiently and uniformly seed small fibrous scaffolds for applications in 3D cell-based assays for high-throughput screening. (c) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010.
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作者简介:5 o6 G' ]% Y. d% H# g
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杨尚天教授是美国俄亥俄州立大学化学工程与生物分子工程系教授,长期从事生物工程和生物加工的研究和开发,特别是新型生物反应器的研发、生物催化、发酵、细胞培养、组织工程、生物芯片、生物传感器等方面具有很深的造诣。担任5个国际学术刊物的编委或顾问编辑和4个国际著名刊物的审稿人。历任多个国际知名研究院或大学的客座教授。同时担任美国农业部、美国能源部、美国化学协会石油研究基金会、香港研究基金委员会等项目评审专家。现任美国国家健康研究院(NIH)生物工程科技纳米技术研究部顾问团成员。近五年,发表SCI收录论文41篇,EI收录35 篇,出版学术专著14部,承担的科研项目24项,科研研经费达450万美元,获得美国专利2项。曾获美国中部华人学人协会突出贡献奖。2007年,当选美国医学和生物工程院Fellow。 |
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