人骨髓间充质干细胞在成年大鼠脑内的迁移及分化

cdk1

2 E; `1 a+ v' D4 j: {1 k# Z
2 ?; d3 M; }- [% r' W' ?" H. [侯玲玲1 , 郑　敏1 , 王冬梅1 , 袁红丰1 , 李海民1 , 陈　琳1 , 白慈贤1 , 张　涌2 ,
3 O/ x1 w" N9 B2 ?3 p7 V裴雪涛1 , 3' f: f' g7 i5 H+ H$ l
1 军事医学科学院输血研究所、军事医学科学院干细胞中心, 北京100850 ; 2西北农林科技大学胚胎工程实验室,
. X. n  Y  ^% Y0 \& d陕西省杨凌712100
. y) A9 e% c2 `1 p# _2 O/ e摘　要: 　骨髓间充质干细胞(mesenchymal stem cells , MSCs) 是目前备受关注的一类具有多向分化潜能的组织干细胞,
% h% U7 R: z; i2 `  r体外可以分化为骨、软骨、脂肪等多种细胞。因此, MSCs 是细胞治疗和基因治疗的种子细胞之一。为了探索MSCs 的. @4 X  S1 c* u
迁移和分化趋势, 为帕金森病(Parkinson disease , PD) 的干细胞治疗提供理论和实验依据, 本实验将体外扩增并转染增+ Z3 I3 h" h! R7 ]  q: J
强型绿色荧光蛋白(enhanced green fluorescent protein , EGFP) 的人骨髓MSCs 注入PD 大鼠脑内纹状体, 观察了人骨髓) ?. w) P7 \) U, C
MSCs 在大鼠脑内的存活、迁移、分化以及注射MSCs 前后大鼠的行为变化。结果表明, 人骨髓MSCs 在大鼠脑内可存
( X1 ^6 Y; G4 Y. a活较长时间(10 周以上) ; 随着时间的延长, MSCs 迁移范围扩大, 分布于纹状体、胼胝体、皮质以及脑内血管壁; 免疫
$ |& E$ H  M# l; {8 y组化法检测证实MSCs 在大鼠脑内表达人神经丝蛋白(neurofilament , NF) 、神经元特异性烯醇化酶(neuron2specific eno2' g/ R  I: F) l7 T( ?& U. s: H
lase , NSE) 以及胶质原纤维酸性蛋白(glial fibrillary acid protein , GFAP) ; PD 大鼠的异常行为有所缓解, 转圈数由8186 ±
8 j" C9 W# i0 ~) q, K7 Q2109 r/ min 下降到4187 ±2106 r/ min , 统计学分析P < 0105 为差异显著。以上观察结果表明, 骨髓MSCs 有望成为治疗
+ ~" _% A( B$ {" @$ ~0 Z. SPD 的种子细胞。) k9 `; @8 L) P( R) `  _) M% @8 x
关键词: 骨髓间充质干细胞; 大鼠; 脑; 分化; 迁移
5 m( w0 W( d4 J, }: T& I中图分类号: Q254
8 U) T+ a1 b* \/ L. T( gMigration and differentiation of human bone marrow mesenchymal stem& h4 G8 x" U7 ^9 p! L# y
cells in the rat brain2 S5 T3 r, Q  J7 z: J& h6 F$ L
HOU Ling2Ling1 , ZHENGMin1 , WANG Dong2Mei1 , YUAN Hong2Feng1 , LI Hai2Min1 , CHEN Lin1 ,. ~- I$ b/ p- Q! c" t; \1 J$ Y
BAI Ci2Xian1 , ZHAN G Yong2 , PEI Xue2Tao1 , 3
$ X0 K! Z& C; B$ |: s- S1Beijing Instit ute of Transf usion Medicine , Beijing 100850 ; 2 Northwest Sci2Tech University of A gri2# L% S6 x9 W% v% R
cul t ure and Forest ry , Yangling , S hanxi 712100  T, H3 x" A) L* u' @/ O
Abstract : 　Bone marrow mesenchymal stem cells (MSCs) are multipotent tissue stem cells that can be induced
0 r8 s# j8 r( B! n$ [in vit ro to differentiate into a variety of cells such as osteoblasts , chondrocytes and adipocytes. MSCs are useful
. G% q' K5 R, @9 r/ ~: ?1 r/ H% X: l7 ^% [+ a& v1 D
) a9 K1 p" q/ T# F# I
vehicles for both cell and gene therapy for a variety of diseases. Here , we injected human MSCs with enhanced
' i7 i& i2 Z; K( L3 l8 s; w; Cgreen fluorescent protein (EGFP) into the striatum of Parkinson disease (PD) rat and examined their survival ,  V, U+ w* N( I" g- v
migration , differentiation , and the behavior changes in PD rats , which will provide a theoretical foundation and
5 B; o2 Y4 g' a  g: C3 _4 {technical method for clinic PD therapy by stem cells. The results showed that human bone marrow MSCs can* r+ U  ?$ n& z/ ?
survive in rat brain for a long time (exceeding 70 d) . MSCs were found in multiple areas of the rat brain includ2# c. M& z* U# D2 N
ing the striatum , the corpus callosum , contralateral cortex and even the brain vascular wall. Immunocytochemical
' Z; r- R: l1 Estaining suggested that implanted cells expressed human neurofilament (NF) , neuron2specific enolase (NSE) and
: X3 e' A+ z% _7 {/ iglial fibrillary acid protein (GFAP) . At the same time , remission in abnormal behavior of the PD rats appeared.$ H. c9 \0 w4 t* {
Rotation scores decreased gradually from 8186 ±2109 r/ min pre2transplantation to 4187 ±2106 r/ min 90 d post2
, G1 ~% M/ N3 I' ltransplantation (statistic result showed P < 0105)
& Q' I3 Q. o2 r1 y* S0 ^* Q' G
. h" O$ A0 j: t2 ^# L! y7 j& o9 _附件是pdf全文。感谢大家支持，回复本贴就可以下载了2 \& N$ D( M# C

( r; O; Y2 E7 }% r3 m1 A

新人王

非常感谢版主的分享，支持你

horst

真是好资料，很有帮助，谢谢了

22002393

3QXX

茶水

学习

cuicuienya

谢谢

weianh

tspaulzhao

Thanks.

零落幽谷

回复 1# cdk1 , U2 \# s  t! \4 _% ~! E7 c4 d
看看

凤仪飘雪