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MEF里不会是一种纯细胞，但随着代数的增加会变纯
4 g$ [ m* ?& _2 F6 z9 e C3 H4 j. t5 n" |, J( z' ~7 B# s% o% B6 a
最近的一篇文章有没有看过（附件中）, F2 o0 o+ Q- j9 [. f# G; K
Multilineage-differentiating stress-enduring (Muse)1 v. g; ~$ ]8 r5 p8 c* I
cells are a primary source of induced pluripotent) j: [4 A. Q- l* k9 F! Q, h# Y
stem cells in human fibroblasts4 L: e% O0 s, I$ j
Shohei Wakaoa,1, Masaaki Kitadaa,1, Yasumasa Kurodaa, Taeko Shigemotoa, Dai Matsusea, Hideo Akashia, H( ]4 W" C ^
Yukihiro Tanimuraa, Kenichiro Tsuchiyamaa,b, Tomohiko Kikuchia, Makoto Godac, Tatsutoshi Nakahatad,2 o- Z [4 b- m6 x0 c( k; w( A
Yoshinori Fujiyoshic, and Mari Dezawaa,25 f5 h+ m/ b3 ?+ j9 ?
Departments of aStem Cell Biology and Histology, and bDermatology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575," i: M; n# N- t
Japan; cDepartment of Biophysics, Graduate School of Science, Kyoto Univerysity, Kyoto 606-8502, Japan; and dCenter for iPS Cell Research and Application,& `; G0 I) |, F' K9 M
Kyoto University, Kyoto 606-8501, Japan
5 |, ^1 R Y- Z- w* @Edited by Darwin J. Prockop, Texas A & M Health Science Center, Temple, TX, and approved May 4, 2011 (received for review January 19, 2011)" ?* ^! P* ^9 V9 w& d: |3 B% w
The stochastic and elite models have been proposed for the
# D3 }) l2 [4 Dmechanism of induced pluripotent stem (iPS) cell generation. In0 {3 Q7 E7 V/ N) {: S M' D% |4 {% u0 k
this study we report a system that supports the elite model. We
/ Q- F) K; \8 s# W5 z6 i8 S. spreviously identified multilineage-differentiating stress-enduring5 {7 K/ y" S ?& |
(Muse) cells in human dermal fibroblasts that are characterized
( p% A$ O$ t# |% ^. M$ Wby stress tolerance, expression of pluripotency markers, selfrenewal,9 s5 }2 J# ]* E
and the ability to differentiate into endodermal-, mesodermal-,
$ B# X, G5 n' c3 x. Yand ectodermal-lineage cells from a single cell. They can* X) g7 r0 Y% f, f/ V3 \/ A3 T
be isolated as stage-specific embryonic antigen-3/CD105 doublepositive& c. w# E2 v% a; [: U0 j0 G. C
cells. When human fibroblasts were separated into Muse' R& u/ s8 D* O! c0 e% q0 J) B2 C
and non-Muse cells and transduced with Oct3/4, Sox2, Klf4, and6 V1 |/ t% Z, h
c-Myc, iPS cells were generated exclusively from Muse cells but0 ~, Q: u) C# L" q; x+ Q! q
not from non-Muse cells. Although some colonies were formed4 T* X: E5 | m V8 H( L1 |
from non-Muse cells, they were unlike iPS cells. Furthermore, epigenetic& N% H, R, w' `# Z
alterations were not seen, and some of the major pluripotency
) P0 e. ?4 B$ G$ Qmarkers were not expressed for the entire period during
4 X) P# Y6 Q& E ^iPS cell generation. These findings were confirmed further using4 v; X# q$ Y- N
cells transduced with a single polycistronic virus vector encoding
! j+ [7 ?( d0 \. t8 \- _$ i3 Oall four factors. The results demonstrate that in adult human fibroblasts
* S# r7 e3 O8 J8 Va subset of preexisting adult stem cells whose properties are
2 Q v/ R3 n* C6 g) f# V9 ~4 psimilar in some respects to those of iPS cells selectively become iPS8 G: X. H9 N4 h1 W! w4 }5 }
cells, but the remaining cells make no contribution to the generation4 M+ e2 Z$ q9 c7 L7 c& ?
of iPS cells. Therefore this system seems to fit the elite model' q# `6 ]: |1 t# l, O
rather than the stochastic model.+ }' n+ l/ t# z/ l; y6 T
9 o' A6 } k+ s% Q' E) w在原始Fibroblast中用特定Marker分离出一群细胞，只有该种细胞可以被诱导为iPS，基本可以回答你的问题吧，MEF中可见一斑 |
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发表于 2010-7-6 22:15
