悬浮培养干细胞所用培养液中FGF-2和EGF最终浓度是5ng/ml,还是10ng/ml?

mhm

The cells were then inoculated into N2 or B27-supplemented RPMI-1640/1% methylcellulose medium without serum at a density of 1x105 cells/well in ultra low attachment six-well plates N2 medium consists of 2X DMEM/F12 supplemented with progesterone (20 nM), putrescine(100 μM), sodium selenite (30 nM), transferrin (25 μg/ml),insulin (20 μg/ml), and human recombinant epidermal growth factor (EGF; 10 ng/ml) and basic fibroblast growth factor (bFGF; 10 ng/ml) mixed with an equal volume of 2% methylcellulose.
0 V) e3 G5 }$ p: O% @7 G' K- c; c EGF，bFGF是和DMEM/F12混合后浓度为10ng/ml，然后再和等量的2%methylcellulose混合？还是和等量的2%methylcellulose混合后最终浓度是10ng/ml？

qiuweiqin0618

应该是终浓度

manybit

为什么要悬浮培养

mhm

谢谢qiuweiqin0618！最终浓度是10ng/ml。

mhm

manybit，悬浮培养是从肿瘤细胞中分离出干细胞样细胞！