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N-2 Supplement (100X) liquid
( `, i% w" V$ r8 r' _! a! ^5 S
) c; h4 j3 O" C' dFor the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype.$ j- Y; F. Q/ |5 M* k
+ b. f9 k) `5 B! Q" D; ?N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation (1).This supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).It can be used as a substitute for the N-1 Bottenstein formulation.N-2 medium appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines.(in Current Methods in Cellular Neurobiology:Volume 4, edited by Jeffery Barker, John Wiley & Sons, Inc.,1983.)
: ?+ s+ V6 Q. j
I6 E9 ]6 ^$ G# q' G1 b) QCatalog Number(s):17502014 ,17502030 ,17502048 ( o0 v* }6 h1 o. c4 F4 s! c
COMPONENTS 分子量 Concentration (mg/L) mM % Y2 ~! k/ q: f/ [4 t3 m$ j
蛋白质
( v' F9 R4 d) {Human Transferrin (Holo) 10000 10000 1- h5 @0 A% N* z, P
Insulin Recombinant Full Chain 5807.7 500 0.08611 [- N2 S" I: D6 f) c
Other Components) u3 m$ n- D4 P% Z( l/ C8 B
Progesterone 314.47 0.63 0.0028 D# Z: e3 R. K; {+ @& h
Putrescine 161 1611 10.01
4 L- o0 X0 W$ E4 m8 ? ~7 W6 W( zSelenite 173 0.52 0.00301
1 U; c3 z& ?$ _ [ |
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