PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页* l. c, B5 q! p+ |/ j

; d6 l, ~+ `* L$ k# ?( L6 X; ~( t' QOnline ISBN:9780470151808
! }. X' L7 A. [2 j: D0 oDOI: 10.1002/97804701518086 z7 I6 k% W1 I- P' K& |4 p
Table of Contents5 [0 F9 z  f# Z: ^
1. Foreword
8 o( b2 I3 F- U; r$ G! }2. Preface
9 O' [! F8 _( x+ o' V3. Chapter 1 Embryonic and Extraembryonic Stem Cells' ^9 M) E. f2 e) A# P
1. Section A Isolation of Embryonic Stem Cells
  j/ N' X+ E% \5 _1. Introduction
; W& B+ {6 R( v$ B' q) _: D2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
4 _+ m9 K2 q- l% K7 Q2 A8 ?( L3. Unit 1A.2 Derivation of hESC from Intact Blastocysts1 _7 s* O& c  |4 J$ _# N
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
2 r1 ^/ D$ a. u' ^5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
( o, ?3 C% z( `; e+ }2. Section B Characterization of Embryonic Stem Cells
' l7 S7 |- U7 Y1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
! X# G9 C' Q) I2 R6 f3 I# |0 B" x2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
/ }3 k% Z( v& W& w4 K" Y5 gEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform. y5 g+ v$ v9 m. Z+ B6 {6 Y! l% Q
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells/ |2 v2 t5 N4 D- q; t
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of2 K" w1 |* M5 g/ x
Pluripotency* i& r, T& m% c
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells6 g1 i7 {0 }. t- }+ l' h' g$ x; m' O
Using In Vivo Biotinylation7 H8 q6 [( L7 O- M, o( C
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem
" I: E# T7 @# ]; w; K2 bCells3 E" E9 d: e! x* u! o% N
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional1 g1 T  E' T1 i
Profiling2 i' r* F% [" p3 h: Y
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells$ }& q4 f9 U7 I
1. Introduction1 F/ @- g% A: q) n0 `4 e$ @
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
- a( d) A- D. o' w0 B3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture" D& I; J: s8 I1 L
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
. v" u9 l) @) ~& ?1 oMouse Embryonic Feeder Layer Cells/ i) }5 G2 |4 |0 e& f
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
- \- b& w5 e8 r+ x6 X+ i3 u6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic5 A9 y* B# T! U, Z  j
Stem Cells
) n2 c' ]6 Z# {7. Unit 1C.6 Isolation of Human Placental Fibroblasts
  [" M2 m' i/ s8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic' W  }3 \& Q/ x( p' d
Stem Cells/ S" N: ]" n5 t1 Q
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of% }6 x- N0 H6 F7 u4 T) W
ROCK Inhibitor4 M6 J. @& I( ~6 s9 C3 G: w
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
* c/ |" z# D% D( R- E, h7 \11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent7 X5 l0 u& f# _
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
6 ^0 ~+ D# {1 O3 A' B12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers* h) U# Q  W2 r1 T2 I, n( f" }
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells4 M- }- @1 L8 T* |
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
6 H# T' S. y) U7 |( o" Q2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
  ?6 @3 S& T5 D$ N, PSuspension and Hanging Drop Cultures
$ S; `4 x5 a6 s% e- K  B3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
$ P/ e! h/ M5 M% l3 Aand a Description of the Hematopoietic Blast Colony Forming Assay
# l9 Z1 k8 l- V) n4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically' m' g1 }2 T: i* G: p
Defined Culture Conditions
+ S0 ^( w* S" X6 ?% k8 F9 q5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
" n$ M, f$ G( |& {5. Section E Extraembryonic Lineages7 _  o+ h" I2 O3 o8 z
1. Introduction
) I- ]# }; q0 z# B5 U, m. q2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
& N$ g/ [  [1 r$ J% X7 ointo Hepatocyte-Like Cells( C/ Z( M( b+ C7 r& p9 }% r
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
7 F/ Z/ d( H7 j- a. W4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells4 ?* V  V9 [- m) H) t: j% x+ `/ c
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells  H" a" b: S6 P" @; k
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells6 j3 S, V6 ]0 {2 C
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use/ x( F5 u1 o# b' E1 m/ _
6. Section F Mesodermal Lineages6 `$ T- O) ~% |% H% [6 D% l5 I
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells$ e- |( Z7 _9 J3 s" [' J: I' ]
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture. O2 B' ^& m0 s/ h0 O; I  I8 B
with Endoderm in Serum-Free Medium# L2 `7 R. Y0 ]* f
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
5 H7 K/ U' l* L: j1 |4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
7 y  t1 l! o2 ~# R% o, H$ ?4 E9 ^5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
3 D- ?& B. U6 l6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation: C% o- Y! N- N+ g- a8 b
with Stromal Layers5 u1 n& O( j8 Y8 o8 L4 p2 o
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary: Y# O! R5 z8 J2 y; }% {( F
Murine Hematopoietic Progenitors
' ~5 s4 ~% s8 d. J3 s8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from! c* N, p0 {% A+ C7 I* O
Human Embryonic Stem Cells
- E4 Z. T- U& s- G3 o  Q9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells9 K- E' ~, P/ C8 x7 n
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac' J, L$ q$ }/ M  G/ a- z. w
Progenitor Cells
, Z) Q) s* I6 D4 g. h11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the# Y  I* l2 M; X" L% k
Hanging-Drop and Mass Culture Methods
, }2 N0 P# e- J# L$ X- A7. Section G Endodermal Lineages
* `- U+ [1 g4 q7 O, J  {1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells1 q/ w  o+ U. \6 ?0 |4 m0 A1 k
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
& [+ T% w3 c8 l, k4 F) E3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm  @8 I7 m% [6 o' i' ?7 h6 W
8. Section H Ectodermal Lineages  v9 h; G! M( w1 G% x& g% L
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons' I. o8 V; L' T  h0 }
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by
4 K4 C0 F% p2 V3 @Two-Photon Microscopy0 G+ g( S/ ~% h8 c! @1 S4 L9 ]
4. Chapter 2 Somatic Stem Cells
: j% K  L# t+ a/ o1. Section A Hematopoietic Stem Cells% O# Q- l7 s* m0 M, V2 w5 c" a
1. Introduction' v4 Z2 U3 d/ {, a1 X" A7 s4 y
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density% w9 A- X5 o/ D$ `3 [
Gradient
& o5 `1 g, [5 d3 `8 |% m/ b' y3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood6 o( Y5 F4 [( r, w
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood5 d0 s  y! @& h: y" ^
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells% w  \/ S' r+ ?% [2 q
from Adult Mouse Bone Marrow6 L0 Q6 v7 O+ A6 n1 r* U& f4 r; n" a
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche% z, D/ T/ n  [+ \
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine% H5 j! @9 ^' ~9 y
Embryonic Tissues
  ^3 s! o. u" D# U% H8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
6 u* N8 |7 D7 T1 ?( k3 P; O9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells; ~, n$ |( m# t) G# Y$ @
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
. @0 m$ @- _6 f2 O! N' h% A! V2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
% ?& O4 W4 o9 _  z2 }4 a; w1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human$ l$ o+ p+ I! n" Z' o
Tissues1 U3 L2 @7 `4 W2 s! z% q" b% J6 L' y
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
" d8 i: [6 U  ]% z$ v1 Y7 F3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells9 A  s6 p- ]1 x
3. Section C Cardiovascular Stem Cells% M8 I: t  A  S/ l
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood5 k7 X0 h3 S) G/ h5 n0 M
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
$ D% l3 V2 X6 k: S. ?# C" h$ [, R, e3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
0 k6 O9 U3 z& ^! P3 Q+ _  o4. Section D Neural Stem Cells
$ v3 D8 v) J3 U+ A4 o# N1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains8 M# L( B* f' Z' h$ }8 w
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells* c" ?6 |3 u0 H
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and* s6 k4 R- @3 K0 u' V3 J
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
' G" n* {( B' W) C  J( i; X. C0 b4 dForebrain Neural Stem Cells8 {! ^  a3 ]* P4 R1 o
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage
' y+ g& w3 W5 ~( hProgression, and Maturation
  j8 n1 d* q- e$ B( U5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic9 u& y- T, E  n2 x  x8 \
Neurons from Rodent Brains- C7 H* }0 Z8 [3 p
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
' t, Z. x1 N% w6 T+ ^( {Technique
/ `- N- j; G: _5 i: W- F5. Section E Germline Stem Cells% P: F5 S1 o6 V4 C) X5 v
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila& G9 R4 d5 v+ {- \* \
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis. n; m5 a# A1 R$ v& H
6. Section F Gut Stem Cells
0 _# j1 P( o0 K1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells3 K1 K0 q% K2 v7 i& E: a
7. Section G Lung Stem Cells
3 s8 V( p# N6 N2 K5 O7 v  x' J1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
% S0 q+ L* }3 F/ i: b5. Chapter 3 Cancer Stem Cells0 v6 s+ E) A- v- |3 ~: A
1. Unit 3.1 Colon Cancer Stem Cells6 U* h  J8 A6 ^/ @% t: S. }! y
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model- J: S4 y8 v0 ^2 @0 h* G$ t, b2 S
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells& T+ U5 j* \9 L0 m/ W) @! O  j$ M
6. Chapter 4 Manipulation of Potency
+ H# O5 w7 }0 ]0 y/ m1 `: s9 a. l& Z# X1. Section A iPS Cells
/ p) s. a! i3 i5 M+ O3 G% b5 o1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming' S8 M8 T7 q2 r: \8 c
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
: ]- ?' |9 J  n2. Section B Nuclear Transfer
3 L  M$ i& G, H5 G& h- }/ a1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
( v. W' e7 O4 t+ j% g* B2 [: b7. Chapter 5 Genetic Manipulation of Stem Cells5 i; K) F  e7 q* |7 }! R, J
1. Section A Lineage Tracers in Stem Cells
; @! J2 m3 }; {! p' Y9 T# Y1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
" x/ W) J, a5 m9 F2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing2 j8 ^7 k4 \; B% g7 L7 P
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells& D( b  n: M. j: Q" z
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
+ r; C- ], ?. |0 d5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
. H( M- g2 Y" s2 F: B2 cNeocortical Neural Progenitors
3 G9 F" M" y- H: U2. Section B Homologous Recombination in Stem Cells- R; M' u+ R% a$ V
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by% M+ c* _4 {) R) O1 ^9 O1 E
Homologous Recombination( B1 D- n0 ]. H+ g5 o  O
8. Appendix 1 Useful Information5 f8 G+ U) V& o  Z# `. U
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research( Z0 K5 U3 q$ b3 w
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
2 Z6 i% T& w# C  |. _9. Appendix 2 Laboratory Equipment( i. `. I* I" ~# V
Standard Laboratory Equipment$ i/ p5 L5 r; E/ X( y+ B
10. Appendix Suppliers
: m3 x9 L# g. G/ R  LSelected Suppliers of Reagents and Equipment+ |9 D) V* u7 e

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