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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑
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_& N( i4 y8 c- Y附加上目录，以供需要的人参考。: F7 y" F- x$ Z) {$ |; |3 ~
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Contents
3 O6 \, r$ R; S/ u" \xi
- M( V+ I. J: ~Preface ........................................................................................................v
) f8 p1 S; b; {" M( YContributors ............................................................................................ xiii* w0 [3 o$ r* K2 |
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PART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES
. u, F8 D# C4 c- \ M/ ~1 Transduction Technologies
* ^2 ~3 Z. `1 q K; HDavid A. Fluri and Martin Fussenegger ........................................... 37 E ]' a5 _# U0 Z. P8 [
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,- G$ u5 A) W3 j& t9 g
and Otto-Wilhelm Merten ........................................................ 238 f4 q, H1 ]4 o' b7 n4 `, n' a
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
( i' ?: T9 V: ~3 N3 {Alexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93. D# w* f& q" O/ [, F6 r. f5 d
4 DNA Fingerprinting and Characterization of Animal Cell Lines0 C D4 Z$ w2 w) B
Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 1232 ~/ d5 h* {4 ?1 U1 z. n
5 u3 P+ Y9 x. QPART II. BASIC CULTIVATION TECHNIQUES
' Y' e5 u% E3 D0 s9 t6 ^. }, F5 Microcarrier Cell Culture Technology
' l( J& U; _- @2 V) i" lGerald Blüml ............................................................................... 1490 E( n, D6 n: e+ @0 s3 w! @2 w: @3 F
6 Cell Encapsulation4 `) u$ N& y4 u; Q1 J" ]2 p7 X
Holger Hübner ............................................................................ 179
7 n- E# X2 k2 m( t& r7 Tools for High-Throughput Medium and Process Optimization R, B6 d4 H! N* K' K) R4 A
Martin Jordan and Nigel Jenkins ................................................. 1939 ^6 B8 b2 Z- ~3 G5 H0 Q: R% N
$ r% m* y+ L( R; Y8 I) vPART III. CELL CHARACTERIZATION AND ANALYSIS+ [' W# W& g; E
8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205 K( G( _& H w$ ^
9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry4 y5 P* r' x, N+ C
Silvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 223
1 }$ r+ a& i( y! l; n10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
8 \: l' ?3 _! t* h+ }André A. Neves and Kevin M. Brindle ........................................ 239
v, @1 D, P" P11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
+ B- B" q5 _8 M+ h! R7 A$ B4 c* B2 G7 VHeino Büntemeyer....................................................................... 253' B2 J" `' f) G% L
12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation0 G8 Z& m5 l; `: k, ~+ l
Derek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269# k$ u1 _' J5 v* ~
13 Measurement of Apoptosis in Cell Culture) L5 V8 \, F7 E0 h0 k% {8 p8 |
Adiba Ishaque and Mohamed Al-Rubeai ..................................... 285
, h a( O' o6 s! h. u* V) b14 Metabolic Flux Estimation in Mammalian Cell Cultures
* L: ~; V' E0 f% d( s; X: kChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 301
2 z+ D) O, U, ZPART IV. SPECIAL CULTIVATION TECHNIQUES
9 `7 v, G, m% M+ Y" @15 Disposable Bioreactors for Inoculum Production and Protein Expression
$ X5 q3 S2 D, x' y$ _4 _Regine Eibl and Dieter Eibl .......................................................... 3213 ~& L3 X( j* L
16 Hollow Fiber Cell Culture3 O1 j8 P# J2 M8 g
John M. Davis .............................................................................. 3372 F3 u% c* i- J9 Z, e0 O
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors) e' Z* z+ R/ X( [ M0 ~
Ralf Pörtner and Oscar B. J. Platas Barradas .............................. 3539 [+ {% V" \6 Z) C7 [
18 Configuration of Bioreactors
4 P* E& m' P$ \/ q3 ?* mDirk E. Martens and Evert Jan van den End ................................ 371
3 `" \* \$ ]+ Z. t C6 X, l* \3 A
PART V. DOWNSTREAM TECHNIQUES
5 b/ ^* F1 B K8 Z; o; \( G4 F19 Membrane Filtration in Animal Cell Culture5 f, X+ O" h; i
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397
1 p" e5 z1 h3 ^2 F$ ^20 Chromatographic Techniques in the Downstream Processing- a/ `" T& H6 ~% Q4 c+ f
of (Recombinant) Proteins9 R, h+ E9 q" ^1 x4 v0 [
Ruth Freitag ................................................................................. 421
* f4 n! p7 }+ k; _" O) [/ ^PART VI. SPECIAL APPLICATIONS
% G+ v9 z7 D- [+ Y# i21 Vaccine Production: State of The Art and Future Needs( A+ C2 i' K4 r% y+ r' z4 {
in Upstream Processing% I- a/ {5 o _
Yvonne Genzel and Udo Reichl .................................................. 457 |& e$ ]$ G; _3 U( t/ f: k% F" K
22 Retrovirus Production and Characterization
8 {2 @# X; n w) f7 T" S! xPedro E. Cruz, Marlene Carmo, Teresa Rodrigues,* y* Q* z) j! H% I4 D( B0 ?
and Paula Alves ....................................................................... 475: F) L& A1 h% u" \" u
23 Insect Cell Cultivation and Generation of Recombinant( Y( v2 _! N c5 B9 R S
Baculovirus Particles for Recombinant Protein Production
( J6 t7 c6 X- i1 RSabine Geisse ............................................................................... 489
) [0 X7 Y0 E! h! U- Z J/ G! Y! CIndex ...................................................................................................... 509
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发表于 2011-3-31 12:51
发表于 2011-3-31 19:05
