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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑
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附加上目录，以供需要的人参考。2 h9 l( C: X! _ B) q' d5 G! @- h( L
* M' q4 ?, H. V$ _- \; t: z2 IContents
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Preface ........................................................................................................v' U% ?. Z$ O3 O- K! ?
Contributors ............................................................................................ xiii3 k$ @( d5 m+ N& e O X
3 J3 D" v+ f3 ]/ B7 F+ rPART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES
- \+ o4 D6 o% q1 o' b: U- Z1 Transduction Technologies
1 p. k. D( j+ M+ w- b' Y% ?David A. Fluri and Martin Fussenegger ........................................... 36 p) o4 p4 k% q$ O0 e U* @
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,
) Y/ D% v. G6 {6 n& Iand Otto-Wilhelm Merten ........................................................ 23
' \& Q' w+ A; b( w* k3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments' f5 a+ V, L0 m- l1 P3 D* ?1 G
Alexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93' {7 ` e6 D( z2 g
4 DNA Fingerprinting and Characterization of Animal Cell Lines
7 _8 P7 B; Y8 {2 K! }! T: JGlyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123 g; R4 Q4 o$ a) V. o
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PART II. BASIC CULTIVATION TECHNIQUES
7 i8 r3 q s" E7 g5 Microcarrier Cell Culture Technology
5 J. I3 e2 u. y2 IGerald Blüml ............................................................................... 149
$ |5 a5 z7 a" D. \6 Cell Encapsulation: l2 \* b. j; o) J! n
Holger Hübner ............................................................................ 179$ F6 w% Z0 L0 R# G8 A
7 Tools for High-Throughput Medium and Process Optimization
" V: I/ v" g% q, Y' NMartin Jordan and Nigel Jenkins ................................................. 193
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PART III. CELL CHARACTERIZATION AND ANALYSIS) F+ x* P1 F& Z u6 l1 \
8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 2057 ?# [1 r. {3 o/ {( j. W( k* g
9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry. C' C4 I; z9 J' r# s0 N
Silvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 2231 @/ H( o% g3 D! O* J1 n1 T
10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
* w. Z& h- z/ y; d" Z, q/ yAndré A. Neves and Kevin M. Brindle ........................................ 239+ o' }7 F3 O$ `' _* N O: _9 r0 u8 ]
11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
3 s3 a2 _: t7 o+ F _( n8 G) VHeino Büntemeyer....................................................................... 253
2 A1 n' B( {& X. A1 j E+ a12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation
3 E9 I! q* e1 o+ JDerek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269& j' N! Z: [7 r; `% K3 a
13 Measurement of Apoptosis in Cell Culture
" h1 r0 y5 T, bAdiba Ishaque and Mohamed Al-Rubeai ..................................... 285
0 [! @8 n* y* {14 Metabolic Flux Estimation in Mammalian Cell Cultures
( e3 o6 ]# Q9 s" f1 @2 `& DChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 3019 W. ^3 I8 b5 M9 T) i" _. o" B+ i
PART IV. SPECIAL CULTIVATION TECHNIQUES
( T% B5 A" W/ ~. E0 O. `( q15 Disposable Bioreactors for Inoculum Production and Protein Expression. u. M/ x3 f0 O; B) b7 n
Regine Eibl and Dieter Eibl .......................................................... 321* `6 l5 A* h# w' B) M7 B, H
16 Hollow Fiber Cell Culture
$ u* K0 F! O( f: PJohn M. Davis .............................................................................. 337( W. T% c( z1 {5 H; }
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors
$ e% ~6 x P" y2 iRalf Pörtner and Oscar B. J. Platas Barradas .............................. 353
" s8 K. z% B! U9 d, P. g3 L- i18 Configuration of Bioreactors
+ q# c6 Z; o: ?8 D1 o0 ^9 DDirk E. Martens and Evert Jan van den End ................................ 371
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PART V. DOWNSTREAM TECHNIQUES
6 N1 e# G: D: w6 L7 X6 r, D# ?8 H19 Membrane Filtration in Animal Cell Culture
6 Y# v0 O ^2 R k: N( qPeter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397
2 z1 p$ U9 S- {. |' c% l20 Chromatographic Techniques in the Downstream Processing
: \# O* ?' J' |4 o; H+ b5 }of (Recombinant) Proteins# a3 Q2 s' ]0 L& I! q# h
Ruth Freitag ................................................................................. 421
9 a& d/ F/ I, ?5 fPART VI. SPECIAL APPLICATIONS/ \# x5 c8 n" b/ W5 B8 G! j
21 Vaccine Production: State of The Art and Future Needs5 X A- y$ y. O5 j- D3 M( R5 E( s
in Upstream Processing7 y: A" G) N% D5 ~
Yvonne Genzel and Udo Reichl .................................................. 457$ n' B2 a6 m5 ^( _; K
22 Retrovirus Production and Characterization# l, D4 N: ~4 j+ A3 E
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues,4 r5 T' F- F- t8 l8 u
and Paula Alves ....................................................................... 475
0 q" S: S( g/ I. A/ [23 Insect Cell Cultivation and Generation of Recombinant
& r1 @- o: _4 q$ tBaculovirus Particles for Recombinant Protein Production, L6 J. N4 c% P1 c
Sabine Geisse ............................................................................... 489
9 N, M' [* _" IIndex ...................................................................................................... 509
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发表于 2011-3-31 12:51
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