PDF 电子书：Animal Cell Biotechnology, 第2版，2007年新版

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附加上目录，以供需要的人参考。
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Contents
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3 n4 U: v$ t$ {+ Z4 J, F+ T3 nPreface ........................................................................................................v8 v9 d3 X8 ]# R7 G, `+ E) K5 k
Contributors ............................................................................................ xiii" z7 h% |! D& j* o
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PART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES
5 R, X% l7 F4 D) j+ i  G3 C1 Transduction Technologies
# c; A$ g2 _, c  ]David A. Fluri and Martin Fussenegger ........................................... 3; Q; Q' Y* f/ D  B, I# U1 [1 q9 ?
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,
$ o; e  Q3 a4 T! n$ k5 Xand Otto-Wilhelm Merten ........................................................ 23
# k1 g7 }( E5 n3 ~7 c+ E9 k4 `3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments8 `4 \! m4 f% J) Z
Alexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93
6 g" u' U/ ?2 W7 _4 DNA Fingerprinting and Characterization of Animal Cell Lines  b( C8 n1 g8 A) w, `* l
Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123
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/ J. r% g# p; I" P# Q( GPART II. BASIC CULTIVATION TECHNIQUES
0 v/ }7 s' F/ q5 Microcarrier Cell Culture Technology
4 [# p, i; T) k7 jGerald Blüml ............................................................................... 149
+ C, W# d7 P: b) U6 D1 [8 A. [6 Cell Encapsulation2 ]* G2 p: c1 z8 e
Holger Hübner ............................................................................ 179
1 g" w8 u4 L+ l& Y) x7 Tools for High-Throughput Medium and Process Optimization
: X* m7 o, O4 {& Y/ T& ~# T5 N+ wMartin Jordan and Nigel Jenkins ................................................. 193
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2 c1 @" b- `1 s2 F' lPART III. CELL CHARACTERIZATION AND ANALYSIS
# n1 t, [; @4 r" A6 F8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205
8 O5 _4 j4 R8 R3 D- V+ ~9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry9 o. ]  O+ c- d# c: r" V
Silvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 2230 Z4 i) B0 W7 I+ m5 e9 Y
10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
! \6 P3 g. Z) |* TAndré A. Neves and Kevin M. Brindle ........................................ 2397 T- a/ c/ X& ]% N6 g9 g$ r& q
11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
5 \2 t4 b$ u/ B0 Y! NHeino Büntemeyer....................................................................... 253
9 K: I# [0 R% c12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation! l" W2 y! ]5 t! A# g% c5 u# W" |
Derek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269
6 y5 l& Z$ X- w' \% H13 Measurement of Apoptosis in Cell Culture
- \- d, T$ K4 nAdiba Ishaque and Mohamed Al-Rubeai ..................................... 285/ a9 V$ f, E- p" }% @
14 Metabolic Flux Estimation in Mammalian Cell Cultures+ j, J$ v7 p  _) v2 ~$ O
Chetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 301
7 c" k' h. \1 L5 x1 y4 XPART IV. SPECIAL CULTIVATION TECHNIQUES: H; o4 C% V* c7 U0 G" T6 b
15 Disposable Bioreactors for Inoculum Production and Protein Expression4 ^3 p9 A; E% i
Regine Eibl and Dieter Eibl .......................................................... 321
7 t$ D. l6 ]+ }* b16 Hollow Fiber Cell Culture
4 l; T% ]" A; `# v4 t+ UJohn M. Davis .............................................................................. 337
+ V6 I2 v+ ~; j/ ?17 Cultivation of Mammalian Cells in Fixed-Bed Reactors
  B! H" |2 i! H: a# `# T" U/ ^5 vRalf Pörtner and Oscar B. J. Platas Barradas .............................. 353
3 D8 S/ y, K" I; F7 b18 Configuration of Bioreactors3 V! ~3 {' F: ~. W. k
Dirk E. Martens and Evert Jan van den End ................................ 3716 Y6 }" E. w  Z8 M4 ?* C

3 z6 ?5 K8 S/ e% G  H* w. ?# MPART V. DOWNSTREAM TECHNIQUES
* p8 W% [% m1 \( v19 Membrane Filtration in Animal Cell Culture6 @: j7 h& ?6 J5 k7 w8 H! T+ d
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 3978 X% E4 c! S. Z: x: n# N+ v- b
20 Chromatographic Techniques in the Downstream Processing
( Q, M3 S7 Q' p: y0 t2 A) Qof (Recombinant) Proteins
7 F5 o/ N2 P3 ^- `/ C: `5 BRuth Freitag ................................................................................. 421; W- r/ K' m1 A. R8 Y& P5 F' T
PART VI. SPECIAL APPLICATIONS  y% H- ]5 ]& N% M6 B% T, l2 B
21 Vaccine Production: State of The Art and Future Needs0 m# I$ `3 |" e5 X/ H7 x
in Upstream Processing3 w: _8 K+ B. s
Yvonne Genzel and Udo Reichl .................................................. 4573 t, g$ G) Q2 u3 T
22 Retrovirus Production and Characterization9 _7 ~3 h; G1 }' Z& W5 l
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues,1 K) _% a# ?* e% M$ {+ h6 ]8 v/ x
and Paula Alves ....................................................................... 475; j5 d7 d: G" a. ?  ~* g
23 Insect Cell Cultivation and Generation of Recombinant
2 {! @9 M2 n2 a- N! T5 V" hBaculovirus Particles for Recombinant Protein Production
8 ~! d, }$ O% x4 mSabine Geisse ............................................................................... 489% z% K& n" v9 i0 L
Index ...................................................................................................... 509
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