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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑
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4 N* w- Q* m! n% @ X) _附加上目录，以供需要的人参考。$ [5 b& Y% e5 X" I" F
) }$ T6 m" {' \+ kContents# T5 L& y, {! b Q% ]
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* K3 k( i5 W* A+ o0 Y( Y$ CPreface ........................................................................................................v% J: n) S5 t; i( ~3 F5 L) Z
Contributors ............................................................................................ xiii$ D1 u+ n4 ]1 s! W. m+ N
5 ?- x$ \& y- hPART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES
4 J$ X/ i" t3 U" {4 E! J/ i1 Transduction Technologies
) U( f, ]( l1 r4 B: dDavid A. Fluri and Martin Fussenegger ........................................... 3
4 z& n( x- ~4 h2 s1 ?2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,3 I$ i* r1 ]4 S( z+ }5 p1 L" R
and Otto-Wilhelm Merten ........................................................ 239 Q v# O4 ~+ C/ p
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments* b. X5 C! |! N# `0 f
Alexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93
& k9 ]3 o0 {/ }" J! @4 DNA Fingerprinting and Characterization of Animal Cell Lines5 }% P1 Y" q: k! B' F: w) u
Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123
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PART II. BASIC CULTIVATION TECHNIQUES
7 C; Z' ?! E: D: n( ~, b) j. j5 Microcarrier Cell Culture Technology2 [! ~9 n6 _/ L! z! Z
Gerald Blüml ............................................................................... 149
* A3 w4 i4 C- U' \ o6 Cell Encapsulation2 h+ C( Y- F0 u# |7 U0 r
Holger Hübner ............................................................................ 179
6 w8 ?1 z& V1 g8 m, _7 Tools for High-Throughput Medium and Process Optimization0 T" e/ T( z3 y
Martin Jordan and Nigel Jenkins ................................................. 193, j( F2 N! Q B, W' ]5 s0 g
$ n$ v; o3 ?) h$ p" o$ mPART III. CELL CHARACTERIZATION AND ANALYSIS+ J% J% [+ }! V( z! ~( M, j! X
8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205, R8 V6 \. j7 a4 S4 x( k
9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry
7 n, a: d- r$ J. [5 m: m5 `Silvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 223
- m5 Q( w& T+ n/ v; E; f6 E10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
9 x" f( y0 Z( ^! I/ [ }André A. Neves and Kevin M. Brindle ........................................ 239
/ c( p3 u# {- y& C. x6 E' F11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
3 J$ c& \$ x5 `% F5 xHeino Büntemeyer....................................................................... 253) `) h, \. |8 y7 @4 }# k6 C. }
12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation, N( D1 N4 o* @& H
Derek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 2694 s6 R; K& B6 h$ b
13 Measurement of Apoptosis in Cell Culture
' `5 `# |1 ?: M) m- y l) r7 aAdiba Ishaque and Mohamed Al-Rubeai ..................................... 285& {# Z+ Y+ ]4 t, r5 u( O
14 Metabolic Flux Estimation in Mammalian Cell Cultures2 R, V$ b5 y& G0 Y5 J) C- F1 I6 T3 c( j9 v8 l
Chetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 3018 Y( R' O4 j3 d0 a. _7 E
PART IV. SPECIAL CULTIVATION TECHNIQUES I3 a0 m9 C& c/ ?; ]$ R2 {' g
15 Disposable Bioreactors for Inoculum Production and Protein Expression3 O, M8 v+ K! A% l) K
Regine Eibl and Dieter Eibl .......................................................... 321
1 M* ^% K9 C9 O, X) n7 \2 p# ?7 N; X N16 Hollow Fiber Cell Culture* c( U, K! i8 w
John M. Davis .............................................................................. 3373 E. A+ p& [5 m* J8 k
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors+ B! P% N* ^0 U
Ralf Pörtner and Oscar B. J. Platas Barradas .............................. 353
/ T H3 Y) ]8 u* F1 }- r18 Configuration of Bioreactors) v* x& z. \8 x6 h- j
Dirk E. Martens and Evert Jan van den End ................................ 371
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PART V. DOWNSTREAM TECHNIQUES
: ~; H( G/ R8 Y4 I& p; u19 Membrane Filtration in Animal Cell Culture: _+ j/ h' f6 f, u
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 3973 b* a( g$ m* [! X& s% l
20 Chromatographic Techniques in the Downstream Processing
' v* a6 z7 M0 }/ C$ P0 Fof (Recombinant) Proteins f4 J; n/ p# l1 U5 G" K# M7 Z- q
Ruth Freitag ................................................................................. 421
@7 L/ S2 H* K: G3 V$ bPART VI. SPECIAL APPLICATIONS' X$ P7 [# x& k
21 Vaccine Production: State of The Art and Future Needs6 ^/ j3 n$ w B2 A0 h8 Y
in Upstream Processing( v4 f4 K* ?3 y2 m v( o
Yvonne Genzel and Udo Reichl .................................................. 457# `$ P. M' m+ ?) j$ g) B
22 Retrovirus Production and Characterization
! l+ z! ]4 W; Q4 W& E0 ePedro E. Cruz, Marlene Carmo, Teresa Rodrigues,- P) x1 c K4 A% M C+ [
and Paula Alves ....................................................................... 475& K6 h2 U1 U! k
23 Insect Cell Cultivation and Generation of Recombinant
% }( }: O" z% WBaculovirus Particles for Recombinant Protein Production' Q6 \8 L. ]- }- c7 y& A2 W0 \
Sabine Geisse ............................................................................... 489
, ~' D2 ^! F+ e6 p" vIndex ...................................................................................................... 509" F$ ^- [! a: {' |- v c
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发表于 2011-3-31 12:51
