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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑 8 s+ }7 C! k: U3 W
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2 ~. Y7 I0 C. T/ q0 H/ i% s附加上目录,以供需要的人参考。+ z. {9 m4 N+ H2 P' n- h1 D' ^
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Contents6 p# {8 ]! `1 A- z& l8 i' ]3 U
xi
. z3 _/ m; q# r$ YPreface ........................................................................................................v
2 G# o; i% |, A" D& N/ N8 n3 NContributors ............................................................................................ xiii& w% z+ U' [6 ]4 D; ]& l! R
3 e( M/ y5 e; w0 X( Y9 h" V: H$ ~) MPART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES- _6 g% \/ P& _# x
1 Transduction Technologies
& a) N# i' T7 lDavid A. Fluri and Martin Fussenegger ........................................... 39 l; c% Q, i9 b
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,9 B( p# }1 B9 z
and Otto-Wilhelm Merten ........................................................ 23" s3 x1 M; Y; K8 X: a* R: k5 }0 w. C v
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments! g) e! I% Z7 ]3 f' P: x
Alexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93
# ?# q. q k, S0 I# {7 N4 DNA Fingerprinting and Characterization of Animal Cell Lines
0 v4 ^& F; Q J7 K8 [Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123. k5 r: E) t* q! k& M8 e
, m/ Z9 Q- b; x: }PART II. BASIC CULTIVATION TECHNIQUES
3 b3 [5 W" a3 }' f5 j5 G5 Microcarrier Cell Culture Technology
3 A! E$ f" |# I1 x8 N5 t9 F% kGerald Blüml ............................................................................... 1498 I9 T/ V+ A3 Y5 x' ], y. N8 m, w9 I
6 Cell Encapsulation
1 B1 l. ^4 w6 i; C! p# kHolger Hübner ............................................................................ 179
/ j. b3 {1 w- A; Q7 Tools for High-Throughput Medium and Process Optimization
! X" J6 ~; M' g. e# {+ r8 FMartin Jordan and Nigel Jenkins ................................................. 193
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2 H8 A' M$ v& QPART III. CELL CHARACTERIZATION AND ANALYSIS1 n; z7 W0 |: z7 z# I! R
8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205
# f7 e( A5 J3 ^" n6 |& l9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry
?9 ~& ]' O N; [6 B6 B0 YSilvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 223
S& F6 d/ u1 ~; Q; C* T10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
0 u3 _! ~$ D; L4 F# B6 XAndré A. Neves and Kevin M. Brindle ........................................ 239
% p4 ]- E, U! s- O: A: S11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
0 e, B5 M: Q/ MHeino Büntemeyer....................................................................... 253, T) b( v: |8 z7 O
12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation, d8 {- P/ u0 `6 I" y
Derek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269
+ i+ X; g# q e$ b, O$ H: C13 Measurement of Apoptosis in Cell Culture
$ h" ?, O; R9 r- l! ZAdiba Ishaque and Mohamed Al-Rubeai ..................................... 285
/ h t& x6 U/ _' \( q14 Metabolic Flux Estimation in Mammalian Cell Cultures
1 k3 ?; b; x6 p9 WChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 3011 ], ^& b- l: u1 @# o2 [$ {& I$ i
PART IV. SPECIAL CULTIVATION TECHNIQUES# R# V( V) _) y" j* P
15 Disposable Bioreactors for Inoculum Production and Protein Expression R" f& j6 t1 K' U8 _
Regine Eibl and Dieter Eibl .......................................................... 3214 j- m2 I. l7 {3 u+ z
16 Hollow Fiber Cell Culture
/ h/ d& L+ \: o% uJohn M. Davis .............................................................................. 337/ d: f5 H7 ^2 x/ v; ?5 t$ P( \
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors0 p/ f$ G5 ^ k/ v8 w3 j7 p
Ralf Pörtner and Oscar B. J. Platas Barradas .............................. 353& g* j# H+ G P3 c
18 Configuration of Bioreactors
$ c" M0 F2 n/ PDirk E. Martens and Evert Jan van den End ................................ 371+ w$ }! w' T4 @- v J* [" ~+ E
6 ]' t+ P$ R4 i: I' k: TPART V. DOWNSTREAM TECHNIQUES
- c- q- x6 ^; t19 Membrane Filtration in Animal Cell Culture2 }2 |0 L3 o! h: F& T$ K! ?$ D
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397) C. }# l @: x9 n8 S
20 Chromatographic Techniques in the Downstream Processing" g3 l% Y0 i" b1 q* b0 B* i4 P3 \ I4 Q
of (Recombinant) Proteins; o v- B' d) x6 a, b
Ruth Freitag ................................................................................. 421" |* \. B- Q* e$ D! o! o5 _+ s
PART VI. SPECIAL APPLICATIONS& { o- M4 s( r, l$ E
21 Vaccine Production: State of The Art and Future Needs. U2 v+ R) j) \. c) j9 T* m
in Upstream Processing% A7 p* {# D+ v; {2 p8 H/ x k6 |
Yvonne Genzel and Udo Reichl .................................................. 457
4 F2 U' _4 s5 }6 h V22 Retrovirus Production and Characterization& ]" c! H0 i- \8 m/ i) J& A" k/ h
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues,3 y& d7 q" P6 K4 _+ g, D) x
and Paula Alves ....................................................................... 475
, {4 F0 m, b6 w23 Insect Cell Cultivation and Generation of Recombinant m: v: t) E u- Y
Baculovirus Particles for Recombinant Protein Production2 |+ @! N; ]. c- ^
Sabine Geisse ............................................................................... 4896 |/ y4 J4 ^. O
Index ...................................................................................................... 509
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