Bloom's syndrome protein (Blm)-deficient ES cells, 就是Blm基因敲除ES细胞。$ M5 i6 S8 E7 |9 @3 Y6 \6 i
Nature Genetics 26, 424 - 429 (2000) , j9 R4 X7 B' ~Cancer predisposition caused by elevated mitotic recombination in Bloom mice+ J) ]3 Y2 [0 V+ n
Guangbin Luo1, 5, 7, Irma M. Santoro1, 7, Lisa D. McDaniel4, Ichiko Nishijima1, Michael Mills1, Hagop Youssoufian1, Hannes Vogel2, Roger A. Schultz4 & Allan Bradley1, 3, 6
Blm indicates a gene coding RecQ DNA helicase, which deficiency will cause Bloom syndrome in human. The relationship between this gene and Bloom syndrome has been noticed for a long time, but the mouse model was first reported by Dr. Allan Bradley's lab in Nature Genetics (2000). In 2004, two back-to-back reports in Nature indicated that Blm-deficient ESCs coulld be used in forward genetic screening, due to its increased loss-of-heterozygosity (LOH). 0 R7 x8 j8 k2 M8 |. L# e5 |5 S1, Nature Genetics 26, 424 - 429 (2000) # i/ b K" _% e c% g
Cancer predisposition caused by elevated mitotic recombination in Bloom mice., L& C( }0 l. g
2, Nature 429, 891-895 (2004) , X3 y+ ]* I' F6 N' WMismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells.- e ]. z: l3 m
3, Nature Nature 429, 896-899 (2004) / D3 w( Z8 \' \0 i! t4 z2 qGenome-wide phenotype analysis in ES cells by regulated disruption of Bloom's syndrome gene.
发表于 2011-4-12 00:57
