脂肪组织来源的间充质干细胞（AD-MSC）促进糖尿病大鼠的皮肤创面愈合

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主题：脂肪组织来源的间充质干细胞（AD-MSC）促进糖尿病大鼠的皮肤创面愈合
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/ P- E; L$ f6 @: J* Q! H+ d! S- X说明：原文来自Diabetes Res Clin Pract，干细胞之家新闻小组成员deron翻译（转帖请注明）
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翻译内容：7 m; ~3 O/ l& k" \( V1 \
目的( G" V+ N- T. E
干细胞是治疗损伤性糖尿病创面难愈的新希望。本研究的目的在于评估脂肪来源间充质干细胞（AD-MSCs）对糖尿病大鼠模型的创面难愈的影响。
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5 M5 g1 }4 \% ?/ b7 W7 X26只大鼠通过单独腹腔注射链脲霉素的方法建模。6只大鼠作为正常组（non-DM）。糖尿病大鼠随机平均分为两组——对照组和治疗组。六周后，在每只大鼠的背部实施全层环切创面手术。治疗组的创面周围进行皮内注射AD-MSCs。实验对照组和正常组同样操作注射PBS。每隔一天测量创伤区域。创伤愈合结束后，取创伤位置的全层皮肤样本，用体视学方法评估胶原纤维的容积密度、血管长度和容积密度以及成纤维细胞的数密度。
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AD-MSCs加快了糖尿病大鼠的创面愈合速度，但是并没有增加血管长度和容积密度，也没有增加胶原纤维的容积密度。AD-MSCs降低了成纤维细胞的数密度。
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AD-MSCs增强糖尿病创伤愈合速率，可能是另有其它的作用机制，而并非通过增强血管再生或者增加胶原纤维密度的途径。: @% v- y7 b; B% e6 ^  n
原摘要：
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3 w+ R' @0 k7 R0 P+ cStem cells are a new hope to ameliorate impaired diabetic wound healing. The purpose of this study was to evaluate the effect of adipose tissue derived mesenchymal stem cells (AD-MSCs) on wound healing in a diabetic rat model.4 ?! f6 g& K, K. Q: S( l9 R
METHODS:
; Q5 c+ B4 N- p* NTwenty-six rats became diabetic by a single intraperitoneal injection of streptozotocin. Six rats served as non-diabetic (non-DM). Diabetic rats were divided into two equal groups randomly; control and treatment. Six weeks later, a full-thickness circular excisional wound was created on the dorsum of each rat. AD-MSCs were injected intra-dermally around the wounds of treatment group. PBS was applied to control and non-DM groups. The wound area was measured every other day. After wound healing completion, full thickness skin samples were taken from the wound sites for evaluation of volume density of collagen fibers, length and volume density of vessels, and numerical density of fibroblasts by stereological methods.5 o! q2 d" }7 t5 h
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AD-MSCs accelerated wound healing rate in diabetic rats, but did not increase length and volume density of the vessels and volume density of the collagen fibers. AD-MSCs decreased the numerical density of fibroblasts.
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; q6 J* W) G1 }We concluded that AD-MSCs enhances diabetic wound healing rate probably by other mechanisms rather than enhancing angiogenesis or accumulating collagen fibers.
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原文链接： http://www.ncbi.nlm.nih.gov/pubmed/21632142?dopt=Abstract，作者先行贴出电子版，因而没有印刷版，原文尚无。

干细胞狂想

这个原文什么时候出啊，很想看！