齐藤通纪培育出人造精子

ppcl2

年轻的齐藤通纪，在生殖细胞研究上近几年成果不断，差不多是这个领域最耀眼的年轻猩猩
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* A$ U8 x; P. _. l4 s$ f- G& x* [Cell. 2011 Aug 3. [Epub ahead of print]
/ }8 K9 ^7 }$ V# A9 j3 EReconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells./ g, T: {* p8 |9 D  J
Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M./ X6 Z6 v# |% r7 h- O1 V
SourceDepartment of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan; JST, CREST, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
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+ M: z) n  Q7 w# L8 bAbstract3 q- s3 {' Y2 m4 A7 y, P0 a
The generation of properly functioning gametes in vitro requires reconstitution of the multistepped pathway of germ cell development. We demonstrate here the generation of primordial germ cell-like cells (PGCLCs) in mice with robust capacity for spermatogenesis. PGCLCs were generated from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) through epiblast-like cells (EpiLCs), a cellular state highly similar to pregastrulating epiblasts but distinct from epiblast stem cells (EpiSCs). Reflecting epiblast development, EpiLC induction from ESCs/iPSCs is a progressive process, and EpiLCs highly competent for the PGC fate are a transient entity. The global transcription profiles, epigenetic reprogramming, and cellular dynamics during PGCLC induction from EpiLCs meticulously capture those associated with PGC specification from the epiblasts. Furthermore, we identify Integrin-β3 and SSEA1 as markers that allow the isolation of PGCLCs with spermatogenic capacity from tumorigenic undifferentiated cells. Our findings provide a paradigm for the first step of in vitro gametogenesis.% d: a* n/ V. f3 I7 K
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llecstem

觉得和真正的“人造精子”还是有点距离！