求带有VP16 C端78氨基酸残基的质粒

xiao00su

想测试目的基因是activator还是repressor，已经克隆了该基因的C端DNA结合的结构域部分，想与VP16的transcription activation domains和drosophila Engrailed 的repressor domain做成融合蛋白
# p& t3 Z' K9 D/ D( N1 ~请问谁有VP16 C端78氨基酸残基的质粒# c8 F% y( i8 i) T- i! c- q7 {# f
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氨基酸序列（413-490=78AA=234bp）apptdvslgdelhldgedvamahadalddfdldmlgdgdspgpgftphdsapygaldmadfefeqmftdalgideygg
$ F! W* h" U8 V) _/ Q) YDNA序列
6 b2 w5 i+ R1 T0 P; |2 K  M' N1 Qgcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgct( v( |+ f) z( o4 ?) _: k. N3 j
agacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacg
3 P* A/ N2 d7 T5 c! Agcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg
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顺便求drosophila Engrailed N端2-298氨基酸残基的质粒4 x9 c3 m; u0 I' I4 w, G
DNA序列
& F7 ~$ t7 T/ j4 X) ?9 @$ hAtggccctggaggatcgctgcagtccacagtcagcgcccagccccattaccctacaaatgcagcatcttcaccaccagcaacagcagcagcagcaacagcagcagcaaatgcagcacctccaccagctgcagcaactgcagcagttgcaccaacagcaactggccgccggtgtcttccaccatccggcaatggccttcgatgccgctgcagccgccgctgctgcagctgctgctgcggccgcccacgctcatgctgctgcactgcagcagcgcctcagtggcagtggatcgcccgcatcctgctccacgcccgcctcgtccacgccgctgaccatcaaggaggaggaaagcgactccgtgatcggtgacatgagtttccacaatcagacgcacaccaccaacgaggaggaggaggcggaggaggatgacgacattgatgtggatgtggatgatacgtcggcgggcggacgcctgccaccacccgcccaccagcagcagtcgacggccaagccctcgctggccttttccatctccaacatcctgagcgatcgtttcggagatgtccagaagccgggcaagtcgatggagaaccaggccagcatattccgccccttcgaggcgagtcgttcccagactgccacgccctccgcctttacaagagtggatctgctggagtttagccggcaacagcaggctgccgccgcagccgctactgcggccatgatgctggaacgggccaacttccttaactgcttcaatccggctgcctatcccaggatacacgaggaaatcgtgcagagtcggctgcgcaggagtgcagccaatgccgtcatcccgccgcccatgagctccaagatgagcgatgccaatccagagaaatctgctctgggatcc
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