求带有VP16 C端78氨基酸残基的质粒

xiao00su

想测试目的基因是activator还是repressor，已经克隆了该基因的C端DNA结合的结构域部分，想与VP16的transcription activation domains和drosophila Engrailed 的repressor domain做成融合蛋白* }1 d3 w/ R5 _7 _$ |( T# o/ ^$ Z
请问谁有VP16 C端78氨基酸残基的质粒* e! ?8 b' n; S% z

& L0 d3 N: w0 _" x7 G" o氨基酸序列（413-490=78AA=234bp）apptdvslgdelhldgedvamahadalddfdldmlgdgdspgpgftphdsapygaldmadfefeqmftdalgideygg
+ n5 D( D2 v( h! s. X/ J* C$ T( a+ CDNA序列* [; E0 `  k+ [7 \* q: |1 D
gcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgct
" B. _4 {8 t8 r, o' \7 M! Y/ p+ O: pagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacg# K" x/ J4 q% S# k
gcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg
! E- P+ u$ s1 m' S- I3 O
/ ]& l/ X# g' F( z3 a& a2 a顺便求drosophila Engrailed N端2-298氨基酸残基的质粒3 u: `2 t! ?: m
DNA序列
! O1 Y3 _& i- o$ N" }  HAtggccctggaggatcgctgcagtccacagtcagcgcccagccccattaccctacaaatgcagcatcttcaccaccagcaacagcagcagcagcaacagcagcagcaaatgcagcacctccaccagctgcagcaactgcagcagttgcaccaacagcaactggccgccggtgtcttccaccatccggcaatggccttcgatgccgctgcagccgccgctgctgcagctgctgctgcggccgcccacgctcatgctgctgcactgcagcagcgcctcagtggcagtggatcgcccgcatcctgctccacgcccgcctcgtccacgccgctgaccatcaaggaggaggaaagcgactccgtgatcggtgacatgagtttccacaatcagacgcacaccaccaacgaggaggaggaggcggaggaggatgacgacattgatgtggatgtggatgatacgtcggcgggcggacgcctgccaccacccgcccaccagcagcagtcgacggccaagccctcgctggccttttccatctccaacatcctgagcgatcgtttcggagatgtccagaagccgggcaagtcgatggagaaccaggccagcatattccgccccttcgaggcgagtcgttcccagactgccacgccctccgcctttacaagagtggatctgctggagtttagccggcaacagcaggctgccgccgcagccgctactgcggccatgatgctggaacgggccaacttccttaactgcttcaatccggctgcctatcccaggatacacgaggaaatcgtgcagagtcggctgcgcaggagtgcagccaatgccgtcatcccgccgcccatgagctccaagatgagcgatgccaatccagagaaatctgctctgggatcc0 _0 w$ N5 {1 I9 z
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