求带有VP16 C端78氨基酸残基的质粒

xiao00su

想测试目的基因是activator还是repressor，已经克隆了该基因的C端DNA结合的结构域部分，想与VP16的transcription activation domains和drosophila Engrailed 的repressor domain做成融合蛋白8 G( @  \" w4 a* n9 a1 l
请问谁有VP16 C端78氨基酸残基的质粒
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$ b: `2 s9 a1 n- F# w. p氨基酸序列（413-490=78AA=234bp）apptdvslgdelhldgedvamahadalddfdldmlgdgdspgpgftphdsapygaldmadfefeqmftdalgideygg0 s  t8 d" `% Y! {0 H" s; _
DNA序列! G* c" ?; H9 f8 b4 [
gcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgct& K- }8 @# a2 X2 e( u
agacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacg
: n! z2 D; m5 b( i$ D6 }  Wgcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg' ~# G9 R) s" ~( h
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顺便求drosophila Engrailed N端2-298氨基酸残基的质粒+ T, [1 Y" B2 e, Y* _0 d, B
DNA序列
4 i2 b1 G/ }( E- ]; `: FAtggccctggaggatcgctgcagtccacagtcagcgcccagccccattaccctacaaatgcagcatcttcaccaccagcaacagcagcagcagcaacagcagcagcaaatgcagcacctccaccagctgcagcaactgcagcagttgcaccaacagcaactggccgccggtgtcttccaccatccggcaatggccttcgatgccgctgcagccgccgctgctgcagctgctgctgcggccgcccacgctcatgctgctgcactgcagcagcgcctcagtggcagtggatcgcccgcatcctgctccacgcccgcctcgtccacgccgctgaccatcaaggaggaggaaagcgactccgtgatcggtgacatgagtttccacaatcagacgcacaccaccaacgaggaggaggaggcggaggaggatgacgacattgatgtggatgtggatgatacgtcggcgggcggacgcctgccaccacccgcccaccagcagcagtcgacggccaagccctcgctggccttttccatctccaacatcctgagcgatcgtttcggagatgtccagaagccgggcaagtcgatggagaaccaggccagcatattccgccccttcgaggcgagtcgttcccagactgccacgccctccgcctttacaagagtggatctgctggagtttagccggcaacagcaggctgccgccgcagccgctactgcggccatgatgctggaacgggccaacttccttaactgcttcaatccggctgcctatcccaggatacacgaggaaatcgtgcagagtcggctgcgcaggagtgcagccaatgccgtcatcccgccgcccatgagctccaagatgagcgatgccaatccagagaaatctgctctgggatcc0 ?$ N3 U# A. }; P: k

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