StemPro 34 SFM是什么培养基？

yangpan521

如题 以前看文献碰到StemPro
34 SFM培养基和STO培养体系，但具体是指什么？还没搞明白，哪位可以给予详细答案。谢谢

Drturtle

回复 yangpan521 的帖子
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StemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。
' o4 I0 l. }  \8 L  q6 [9 |7 Jhttp://products.invitrogen.com/ivgn/product/106390111 E  b+ d& {0 I6 z

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* Y3 x; b/ K, n' q" @至于STO feeders， 请看相关文献的描述： 5 m% d7 y# u' J+ [
Several alternative cell lines have been investigated
6 |  M/ d" d( Y6 i# Zfor their ability to support existing hESC lines as well
8 J9 Z' `- T' ?' }$ @as being used to derive new hESC lines (Table 1). The$ V5 [( \; ^5 `5 _3 c
mouse embryonic fibroblast cell line,STO, has been used  [$ i# e- e2 C
to establish nine cell lines from frozen blastocysts and3 `& h/ ^; q5 ]* Q) z. N) E4 S) W
zygotes (Park et al., 2004). The advantage of STO cells# |2 G2 V( H# E5 `( p
over primary cultures of MEFs is that, being immortalized,) N+ t* a8 M8 T4 i  I+ c- C
they are easy to maintain and propagate. Human7 p3 E3 A1 [' f0 J& u" D
ES cells cultured on this feeder layer exhibited a similar
# I2 P, F# D+ z. N7 ^0 \  Fdoubling time to those on MEFs and expressed surface- b+ ?( C5 R' s' e
markers as expected. In addition, prolonged culture did* l% j. d) b8 f
not lead to abnormal karyotypes. However, Xu et al.
: [- q0 \2 ~- F* n6 j# o$ }) v(2001) have found increased differentiation when conditioned
" y4 z( l. I% H( ^' Xmedium (CM) from these cells was used in
, Y" V  l) W* T, H' S4 V! Tfeeder-free conditions. Thus, the STO line is not a direct7 `" ?0 U9 j$ F  r" z  c& e$ r
substitute for MEFs but can reduce some of the work. o) z: \( b1 @8 l
load regarding MEF isolation and culture.

yangpan521

非常感谢  受教了