干细胞之家 - 中国干细胞行业门户第一站

 

 

搜索
干细胞之家 - 中国干细胞行业门户第一站 干细胞之家论坛 干细胞行业新闻 JoVE:干细胞培养的"3D”技术【附视频】
朗日生物希瑞干细胞
查看: 16617|回复: 6
go

JoVE:干细胞培养的"3D”技术【附视频】  

Rank: 4

积分
2113 
威望
2113  
包包
11684  

优秀会员

发表于 2012-3-6 14:26 |显示全部帖子
本帖最后由 坤明 于 2012-3-6 14:29 编辑
5 g/ A* d3 z( X7 }+ w' x
" ~6 O8 Z: [, P# g1 E7 _- j" ]3 h* n! g4 C6 v9 e1 k
' v9 G* d! d3 {/ b8 f2 C& d3 M6 Z) p+ \
作者:Beyond 来源:生物谷 2012-3-6 10:25:50 0 02 G6 P" b( i8 ?4 W
$ B/ w2 B5 i0 c
干细胞对修复人体受损组织和器官非常重要。因为在体内,干细胞能发育成任何类型的细胞,科学家们相信他们拥有突破性的新疗法的关键。为了更好地进一步开展研究,维多利亚大学的科学家们发现了一种三维培养干细胞的方法,这一技术成果对再生医学的发展具有里程碑意义。3 M) v2 X: a4 v
5 e9 g* S" P) _0 o
“虽然身体内干细胞是在3D环境下生长和分裂的,尤其是当干细胞分化成机体内所有不同类型的细胞时”,文章作者Stephanie-Willerth博士说:“然而,大量的研究工作仍在2D环境下进行的”。
8 u' v" r) @: I9 T, r! T+ T0 R" i* Y. J/ n0 o5 P
  i$ L/ j2 |* h, m% Z
1 r; W* H- B$ ]$ r4 w
从本质上讲,因为身体是三维立体的,干细胞在3D环境下才能最好地发挥修复功效也是有道理的。将这些干细胞在三维立体条件下培养生长,能更好地模拟机体中干细胞所处的三维环境,有利于研究人员更好的开展研究。3 d: u7 @7 N6 t* N( l$ \
# u1 \3 X% h6 S+ m: B6 A
虽然Stephanie-Willerth的实验室主要关注于干细胞对脊髓的修复,但她认为在三维立体环境下培养干细胞对其他领域的研究人员同样也很重要,因此她将这一技术发表在了Journal of Visualized Experiments上。
  S! e# E7 o; T; y7 ^5 y6 X& x! N/ j- d
# d1 A" m1 m. a: @Journal of Visualized Experiments副主编Meghan Berryman说:“由于干细胞研究发展迅速,完全有必要创建出一个培养体系能更准确地模拟细胞在自然状态下的生长”。(生物谷 Bioon.com)
0 H& O. X+ c1 H' E, F( W4 C
  D1 Z$ |6 }7 c( l. O: k6 _1 J( o  ^& j) h( j. C2 o
Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
: W3 m# F/ q/ [& s) N) C5 f, V. I/ ~2 F4 W# n* p
Kathleen Kolehmainen1, Stephanie M. Willerth2
2 d( {2 D, q$ t3 h
' T7 q* p6 b% G6 I. O+ M1 I3 r: a( z  ]$ n% L2 a' g
Stem cells are found in naturally occurring 3D microenvironments in vivo, which are often referred to as the stem cell niche 1. Culturing stem cells inside of 3D biomaterial scaffolds provides a way to accurately mimic these microenvironments, providing an advantage over traditional 2D culture methods using polystyrene as well as a method for engineering replacement tissues 2. While 2D tissue culture polystrene has been used for the majority of cell culture experiments, 3D biomaterial scaffolds can more closely replicate the microenvironments found in vivo by enabling more accurate establishment of cell polarity in the environment and possessing biochemical and mechanical properties similar to soft tissue.3 A variety of naturally derived and synthetic biomaterial scaffolds have been investigated as 3D environments for supporting stem cell growth. While synthetic scaffolds can be synthesized to have a greater range of mechanical and chemical properties and often have greater reproducibility, natural biomaterials are often composed of proteins and polysaccharides found in the extracelluar matrix and as a result contain binding sites for cell adhesion and readily support cell culture. Fibrin scaffolds, produced by polymerizing the protein fibrinogen obtained from plasma, have been widely investigated for a variety of tissue engineering applications both in vitro and in vivo 4. Such scaffolds can be modified using a variety of methods to incorporate controlled release systems for delivering therapeutic factors 5. Previous work has shown that such scaffolds can be used to successfully culture embryonic stem cells and this scaffold-based culture system can be used to screen the effects of various growth factors on the differentiation of the stem cells seeded inside 6,7.
9 J, W* K& t* y3 z+ _
$ b" Z$ E# _4 m& L

Rank: 2

积分
243 
威望
243  
包包
3240  

优秀会员

发表于 2012-3-6 20:24 |显示全部帖子
楼主有没有原文啊,看起来挺有意思的,可以参考学习一下

Rank: 1

积分
46 
威望
46  
包包
73  
发表于 2012-3-7 10:47 |显示全部帖子
希瑞干细胞
不错,有新意,楼主能提供原文吗

Rank: 1

积分
46 
威望
46  
包包
73  
发表于 2012-3-7 11:13 |显示全部帖子
干细胞之家微信公众号
麻烦楼主一个事情,Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies0 n8 P: g, D- N

6 L8 ^  V. `8 e& Y1 I' Phttp://www.jove.com/search?q=stemcell 这个网上的上述视频,楼主能分享一下吗,谢谢。

Rank: 1

积分
25 
威望
25  
包包
142  
发表于 2012-3-7 11:17 |显示全部帖子
这一技术成果对再生医学的发展具有里程碑意义.言过其实。我们实验室一直在进行3D-fibrin gel 的细胞培养,不说试剂特别贵,就是这种结果也是有争议的。因为这种胶在临床上是用于肿瘤术后对残余瘤组织治疗的。
已有 1 人评分威望 包包 收起 理由
细胞海洋 + 5 + 10 欢迎参与讨论

总评分: 威望 + 5  包包 + 10   查看全部评分

Rank: 7Rank: 7Rank: 7

积分
1354 
威望
1354  
包包
484  

优秀版主 金话筒 优秀会员 帅哥研究员 小小研究员

发表于 2012-3-10 10:01 |显示全部帖子
视频里面那人也不怕染菌,就这么四敞大开地做实验,真先进

Rank: 1

积分
威望
0  
包包
1  
发表于 2016-5-30 16:59 |显示全部帖子
实验室内做的打印
你需要登录后才可以回帖 登录 | 注册
验证问答 换一个

Archiver|干细胞之家 ( 吉ICP备13001605号 )

GMT+8, 2019-2-20 20:39

Powered by Discuz! X1.5

© 2001-2010 Comsenz Inc.