大家好，我想培养有核红细胞，请教！！！

yaolinzhang

不知用gibco的STEMPRO-34SFM Complete Medium还是stem cell 的SFEM好？

xieyuhuan

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你好，我想问下你是怎么培养的有核红细胞，我现在也想做这块，求指导，谢谢！

yaolinzhang

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& _/ D9 U# ?/ M4 K4 u% a之前计划做，但最终没有做。不过我可以给你提供几篇文献题目（我没有原文）：
5 }  C% c; y2 y/ @7 `$ H1.Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells
& U$ ]8 o6 b8 P1 z0 u2 C2.Erythroid progenitor cells expanded from peripheral blood without mobilization or preselection: molecular characteristics and functional competence
- M! q, z# j: {+ g$ K6 z8 S2 E3.Red blood cell production from immortalized progenitor cell line6 R0 [. F$ D6 I% N
4.In vitro proliferation and differentiation of human CD34+ cells from peripheral blood into mature red blood cells with two different cell culture systems9 M# R# x. P  i0 Y9 T$ M
5.Ex vivo production of human red blood cells from hematopoietic stem cells: what is the future in transfusion?
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xieyuhuan

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  h6 |$ F4 g( O4 x8 M. u有些文献我看了，就是每个实验室的条件不一样，所用的方法也不一样，目前正在尝试用EB球法做谢谢！