|
- 积分
- 34
- 威望
- 34
- 包包
- 473
|
本帖最后由 细胞海洋 于 2012-6-30 23:50 编辑 8 m Y0 {2 z6 g8 f$ @
6 D3 o8 I% W" ^4 P% oSummary
+ k) }- v6 V4 yIn this report, we demonstrate that an opticcup structure can form by self-organization in human ESC culture. The human ESC-derived opticcup is much larger than the mouse ESC-derived one, presumably reflecting the species differences. The neuralretina in human ESC culture is thick and spontaneously curves in an apically convex manner, which is not seen in mouse ESC culture. In addition, human ESC-derived neuralretina grows into multilayered tissue containing both rods and cones, whereas cone differentiation is rare in mouse ESC culture. The accumulation of photoreceptors in human ESC culture can be greatly accelerated by Notch inhibition. In addition, we show that an optimized vitrification method enables en bloc cryopreservation of stratifiedneuralretina of human origin. This storage method at an intermediate step during the time-consuming differentiation process provides a versatile solution for quality control in large-scale preparation of clinical-grade retinal tissues. |
|
发表于 2012-6-30 09:06
发表于 2012-6-30 23:50
