日本研究发现移植环境会影响神经干细胞分化（附原文）

坤明

& y: ]  W" ^/ u# n5 i

+ V2 s: ]8 W+ c6 ^( g0 U新华网东京１０月１７日电（记者 蓝建中）日本研究人员在新一期英国《自然·通讯》杂志网络版上报告说，移植后的神经干细胞能否顺利分化，与移植部位的具体环境有关，有时环境不利甚至会导致细胞分化完全被遏制。
' {+ g7 N+ b) [3 O- _- ~2 K/ J. i$ j9 v* ~& ~6 q3 q/ O
    目前，利用诱导多功能干细胞（ｉＰＳ细胞）进行细胞分化治疗某些疾病非常受关注。但由于移植部位的环境对细胞分化的具体影响一直没有弄清，ｉＰＳ细胞的临床应用仍存在不小障碍。
3 E' l' q1 O2 |: z/ Y4 y, U! K9 m" E) G( t# U- L' }- y% ]
    日本九州大学冈田诚司率领的研究小组在实验过程中，从小鼠的胎儿脑内取出神经干细胞，然后分别移植到正常的脊髓、受到损伤的脊髓和在试管内进行培养。一周后回收了这些细胞。
9 \$ d, R; l& R$ v4 S. U/ G/ z9 v4 I) m* m0 z. v- K! h
    结果发现，这些细胞中负责分化的基因发挥作用的程度都不相同，特别是在受到损伤的脊髓中，基因的功能整体受到遏制，几乎没有分化为神经细胞。" |, U4 j( _7 [3 p2 z" o- d

' W; Y& M! h8 b6 v    冈田诚司认为，对细胞来说，“如果被移植到过于严酷的环境中，细胞自身为了保护自己，分化就受到了遏制”。研究小组认为，这一研究结果将使人们更深入了解干细胞移植。3 N) t- q! ]% n  S. F6 @2 I

4 N' E9 n1 B* Z# [. i5 F+ l/ O3楼原文 感谢ivantanqi 提供

naturalkillerce

回复 坤明 的帖子& s- c7 @2 d+ B4 p* D
/ m" @5 v2 U8 W, D% I$ b
Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells
4 M. k7 C7 n$ e( z, e( d" [
& J- N- b, E, b9 Z/ D9 f3 ?Nature Communications 3, Article number: 1140 doi:10.1038/ncomms2132
6 b4 o! ~' x) g9 L& u& m
) U+ ^8 J" n1 p! r" mAbstract
! f2 M# L( a  R% e( p4 A7 V1 J2 zNeural stem/progenitor cell (NSPC) transplantation is a promising treatment for various neurodegenerative disorders including spinal cord injury, however, no direct analysis has ever been performed on their in vivo profile after transplantation. Here we combined bioimaging, flow-cytometric isolation and ultra-high-throughput RNA sequencing to evaluate the cellular properties of engrafted NSPCs. The acutely transplanted NSPCs had beneficial effects on spinal cord injury, particularly neuroprotection and neurohumoral secretion, whereas their in situ secretory activity differed significantly from that predicted in vitro. The RNA-sequencing of engrafted NSPCs revealed dynamic expression/splicing changes in various genes involved in cellular functions and tumour development depending on graft environments. Notably, in the pathological environment, overall transcriptional activity, external signal transduction and neural differentiation of engrafted NSPCs were significantly suppressed. These results highlight the vulnerability of engrafted NSPCs to environmental force, while emphasizing the importance of in situ analysis in advancing the efficacy and safety of stem cell-based therapies.' c+ r! D: P2 l

7 B* ?' }- o2 J' O  nhttp://www.nature.com/ncomms/journal/v3/n10/full/ncomms2132.html

ivantanqi

FYI

翅膀111

个人觉得这个文章很有意义啊，不过之前我们类似想法的研究被日本人给鄙视了。