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Rapid Attachment of Adipose Stromal Cells on Resorbable Polymeric
! h* B6 f4 {) @Scaffolds Facilitates the One-Step Surgical Procedure for Cartilage and; Z& z% W* P+ r5 x# C5 c3 K0 f
Bone Tissue Engineering Purposes' C4 d5 w* k0 n. V$ v4 X+ w
Wouter J. Jurgens,1,2 Robert Jan Kroeze,2,3,4 Ruud A. Bank,2,5 Marco J. P. F. Ritt,1,2 Marco N. Helder2,34 S" @4 h1 ~7 a$ U: V. M# N4 M: h
1Department of Plastic, Reconstructive and Hand Surgery, VU University Medical Center, Amsterdam, The Netherlands, 2MOVE/Skeletal Tissue
+ p1 T7 h6 E$ N& S, cEngineering Group Amsterdam (STEGA), Amsterdam, The Netherlands, 3Department of Orthopaedic Surgery, VU University Medical Center,
2 `( ~1 X1 \1 j8 b: [) B4 y$ R. vAmsterdam, The Netherlands, 4Department of Oral Cell Biology Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam& T( S3 i' b6 F J, T8 Q* g. K
and Vrije Universiteit, Amsterdam, The Netherlands, 5Department of Medical Biology, University Medical Center Groningen, Groningen, The' I, B+ {+ A9 y! x5 p3 O2 f
Netherlands
$ c' m, d4 b" W( Y& ^3 A* Z. ~Received 3 May 2010; accepted 8 October 2010! x2 N8 J q5 s/ ~$ p
Published online 18 January 2011 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/jor.21314$ `8 V0 B7 h% q8 Q
ABSTRACT: The stromal vascular fraction (SVF) of adipose tissue provides an abundant source of mesenchymal stem cells. For clinical
4 W4 q$ i7 M2 `% c0 {! d( N# E7 Q' ^; iapplication, it would be beneficial to establish treatments in which SVF is obtained, seeded onto a scaffold, and returned into the patient
& r' `' j) m8 t q( \within a single surgical procedure. In this study, we evaluated the suitability of both a macroporous poly(L-lactide-co-caprolactone) and a& }4 `4 y6 W$ T( ~
porous collagen type I/III scaffold for this purpose. Surprisingly, cell attachment wasrapid (10 min) and sequestered the majority of adipose
0 p. _0 N9 ^' s; w6 T W5 cstem cells, as deduced from colony-forming unit assays. Proliferation occurred in both polymeric scaffolds. Upon chondrogenic induction, upregulation# P: o$ } f% b. ?$ x
of chondrogenic genes, production of glycosaminoglycans, and accumulation of collagen type II was observed, indicating
- n- d9 W2 p* |; P: o" Jdifferentiation of scaffold-attached SVF cells along the chondrogenic lineage. Osteogenic differentiation was achieved in both scaffold
! P$ t0 f: j" ^6 w5 S; {types, as visualized by up-regulation of osteogenic genes, increase of alkaline phosphatase production over time, and accumulation of bone
9 {: q' d% w2 q7 l- m g* Jsialoprotein and osteonectin. In conclusion, this study identifies both poly(L-lactide-co-caprolactone) and collagen type I/III as promising0 \# J0 O" O9 c4 Z' F
scaffold materials for rapid attachment of adipose stem cell-like (stromal) cells, enhancing the development of one-step surgical concepts6 B0 \# A K8 u% y1 V6 C) P- q
for cartilage and bone tissue engineering. 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:853–' [' ^$ D, X1 a7 M
860, 2011 |
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发表于 2013-1-18 15:06
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