PDF电子书：Spectroscopic Methods of Analysis

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Spectroscopic Methods of Analysis2 ^2 v8 j2 H4 t( [+ V

; z+ Q: N8 g9 j: [% c7 r) |( yContents
8 [7 S( A7 i0 \/ w6 a+ H" VPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
$ o) w, S3 w6 C5 @4 |: P  f2 vContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix2 V0 x% V. U4 o% d. K/ }$ }  ~
1 Fluorescence Lifetime Imaging Comes of Age How to Do It and
/ [0 Z" u3 X9 EHow to Interpret It. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
0 A: F3 H- Y8 NYi-Chun Chen, Bryan Q. Spring, and Robert M. Clegg, l% j9 i8 C4 q1 {' x+ e
2 Fo¨rster Resonance Energy Transfer and Trapping in Selected Systems:
; y1 e3 f$ z" u% LAnalysis by Monte-Carlo Simulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 234 t. ^9 Q! ]) t* y7 s3 t
P. Bojarski, A. Synak, L. Kułak, S. Rangelowa-Jankowska,
& m  e, f2 f9 c  g- h) [A. Kubicki, and B. Grobelna
* F; P; |, ^6 V3 K9 A2 e* h) q3 Molecular Organization of Polyene Antibiotic Amphotericin B Studied. k1 V: w' v% {, [9 [) b
by Means of Fluorescence Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57- [% }, _. m) n9 p% i# U
Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko,
0 L# M/ z7 R& O: }6 S# JZygmunt Gryczynski, and Ignacy Gryczynski; J7 J& [& i3 M. l. `/ U
4 Spectroscopic Probes of RNA Structure and Dynamics . . . . . . . . . . . . . . . . . . . . . . . 67( R9 _% a9 s; R7 C, m
Kathleen B. Hall
% b' r# {3 T4 r& d: u' f4 ]5 Fluorescence Methods to Study DNA Translocation and Unwinding) ^" l- @3 b/ q2 Y. I
Kinetics by Nucleic Acid Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
+ X" R) V' {6 \2 |* z8 U' p) c0 I) }Christopher J. Fischer, Eric J. Tomko, Colin G. Wu, and Timothy M. Lohman) [; L/ m5 x# d- ?. D2 ^
6 Fluorescence Intensity, Anisotropy, and Transient Dynamic
+ }" m* i( N2 M! b+ f0 \: MQuenching Stopped-Flow Kinetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105" O. Q! s* B" L2 F  R8 u" A. B
Wlodek M. Bujalowski and Maria J. Jezewska
" X8 S0 `! Y6 y( w' \7 Using Structure–Function Constraints in FRET Studies
" e$ J( P1 V/ x  {" G+ E' J# p% Z% [* Aof Large Macromolecular Complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
* Q0 A( v+ y/ `Wlodek M. Bujalowski and Maria J. Jezewska% V% t4 V* q1 R3 W
8 Illuminating Allostery in Metal Sensing Transcriptional Regulators . . . . . . . . . . . . . 165% f* f/ z% s( ?& e4 H* S
Nicholas E. Grossoehme and David P. Giedroc
( Y, p$ Q- ^! |% W) k1 T2 b9 Fluorescence-Based Biosensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
9 G6 D. G# Y& j8 W9 d- R  AMaria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella,9 H" N7 @0 C/ A, p) {# f( Q
Claudio Pellecchia, and Sabato D’Auria
- C) l) O! b1 E* E4 z6 A) l8 M10 Metal-Enhanced Immunoassays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217' D7 h' S% s8 Z' D" a
Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko,8 w8 N( h  j8 a1 s4 [* S
Pabak Sarkar, Julian Borejdo, Irina Akopova, and Zygmunt Gryczynski7 Y/ \& G$ x' n  `
11 Initial Stages of Angiosperm Greening Monitored by Low-Temperature
  M/ u0 Y7 Y$ R/ QFluorescence Spectra and Fluorescence Lifetimes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231$ @2 \1 v: |# N8 A, H  ?
Beata Mysliwa-Kurdziel, Anna Stecka, and Kazimierz Strzalka
1 B& ]7 b3 @  j" f2 W( ^, N12 Activation of the Mammalian Cells by Using Light-Sensitive Ion Channels . . . . . . 241
6 e2 k  Q" k& n6 X; FMandy Siu Yu Lung, Paul Pilowsky, and Ewa M. Goldys
1 F" {6 ^8 N+ ^13 Detection of Specific Strains of Viable Bacterial Pathogens by Using RNA5 P5 W' X' \- M1 w* N. G# H( @
Bead Assays and Flow Cytometry with 2100 Bioanalyzer . . . . . . . . . . . . . . . . . . . . . 253
5 X. Q1 Q) Q) l, O1 A* ?, LPhilip Butterworth, Henrique T.M.C.M. Baltar, Martin Kratzmeier,* T& P6 {4 d% ]4 _% l; T' C
and Ewa M. Goldys
) I8 x8 ?  s' H* |( g14 Photosynthetic Antenna Complex LHCII Studied with Novel
4 K3 D2 p- w4 s% i' lFluorescence Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263
7 ~2 q' w9 v% |, ]( N! i5 R: m7 ?Wieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski,
5 x' ~  u$ Z9 @4 n# {+ GZygmunt Gryczynski, and Ignacy Gryczynski
7 `; I5 I8 Y+ {8 o8 G; f15 Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule
4 A6 S2 k. D+ E2 ZFluorescence Spectroscopy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
; y: |5 F: |' r# DGoran Pljevaljcˇic′, Rae Robertson-Anderson, Edwin van der Schans,
+ }! J5 U  a3 b* v" b3 jand David Millar
% i: O1 U% x) ?8 P$ q, F16 Single-Molecule Force Spectroscopy of Polycystic Kidney Disease Proteins . . . . . . 297
& H' e9 w0 ^6 X7 t; tLiang Ma, Meixiang Xu, and Andres F. Oberhauser' {4 s0 k) m4 a& Y: [: K- q
17 Single Molecule Detection Approach to Muscle Study: Kinetics
( g8 F) t& E: S3 G% m" X% xof a Single Cross-Bridge During Contraction of Muscle . . . . . . . . . . . . . . . . . . . . . . 311
# \! h$ K& f$ a6 U! `+ V6 G) nJulian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla,
* Q4 p- A* ~. d6 u8 h( \Rafal Luchowski, Zygmunt Gryczynski, and Ignacy Gryczynski3 N( _5 k6 r$ I- _  E
18 Single-Molecule Optical-Trapping Measurements with DNA Anchored
6 S* C& L. \+ [; e% n/ l# f# `to an Array of Gold Nanoposts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335
, s; T3 ?2 l1 ^& x: dD. Hern Paik and Thomas T. Perkins
3 s; U1 i6 q0 |8 a; n19 Electro-optical Analysis of Macromolecular Structure and Dynamics. . . . . . . . . . . . 357
9 z3 \  |! `  P; Z! YDietmar Porschke
3 D& `' G2 j- V2 b+ Z% p20 Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen
0 l: Z6 M/ a7 D7 h, w8 |* HIntermediates in Cytochromes P450 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375; E/ S5 u( P' B/ _2 J
I.G. Denisov, Y.V. Grinkova, and S.G. Sligar
2 u! b3 y, T: g! _5 t$ `8 IIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .: B" H9 \, U5 i: @# P
. . . . . . . . . . . . . . . . . . . . . . . 393. \& O6 R2 r" b
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