求助一本书：current protocols in stem cell biology(已解决）

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Current Protocols in Stem Cell Biology4 c5 G, C8 S4 b0 D2 M
Last Update: July 07, 2009
+ g1 N# l: P1 g, b0 jPrint ISSN: 1941-7322
2 l$ Y2 S: L7 aOnline ISSN: 1938-8969
0 Q' {7 B+ B7 i, f$ u2 t& P% F- vTable of Contents
! R+ ^( y0 C8 x, RForeword
! L1 T' ~+ ~3 b" ]2 f2 KPreface - e+ M/ A* m0 [; |) G8 l
Chapter 1 Embryonic and Extraembryonic Stem Cells ! H" i, j4 z7 H3 ?1 Q1 ?
Section A Isolation of Embryonic Stem Cells 3 k8 n& v# p( |% C" G# b6 ?, t6 P: @, ]
Isolation of Embryonic Stem Cells
3 a: m( l: z7 k1 u) {UNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells % E3 k% ?) j; d; _
UNIT 1A.2 Derivation of hESC from Intact Blastocysts 5 q+ i3 x9 ~" {8 `# d
UNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
' {- j5 _# r- b/ ?UNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
. R! `- }: G8 h6 e0 ] Section B Characterization of Embryonic Stem Cells
" C' I' Q7 r8 v  K6 MUNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells 1 g9 u5 @6 B5 m% y0 {, C
UNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform
9 D5 L, V4 y3 X) ]" eUNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
2 N9 O! J! K- K$ m& HUNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
7 l$ M" L! q- J) P+ c% ^' {3 LUNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
2 N. Y3 r- ?4 s+ P$ M' _& F/ G' S Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
7 |6 P. k+ H2 W. B* \' g8 cCulture and Maintenance of Undifferentiated Embryonic Stem Cells ; t; M0 {- V+ u( x4 h8 }' e6 D' S
UNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro 1 u  g8 u+ \0 A9 P1 U' q7 [7 @
UNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture # w8 g0 H( ~  D$ W0 C4 Z3 \/ W
UNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
) `1 e4 g+ Z' c( P  g6 R' Q8 DUNIT 1C.4 Culture of Mouse Embryonic Stem Cells
# k1 E6 m8 \3 K/ O$ oUNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
6 v5 Q; [, p% s2 L4 v8 ZUNIT 1C.6 Isolation of Human Placental Fibroblasts
0 i0 `5 H$ K; h: O% T- EUNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
/ L1 h# y6 Z. A) y) k" R( HUNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
5 O4 ~9 F) d  h+ k Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
% I1 }# K; x2 Y, L3 I6 B  X. L: a+ WUNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
+ b  C! o# @. z8 y2 u7 VUNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
9 Y4 \1 x. D& t0 d& y: bUNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay / o0 t. w. ~4 I  [) G5 h" U
UNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
/ l+ Y/ N: E3 F$ d% z& lUNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
2 w7 A  @8 z. N Section E Extraembryonic Lineages ; ]$ R0 \+ g; s* p! w+ t! s
Isolation of Stem Cells from Extraembryonic Tissues
) `" t! e5 {+ j' B- WUNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells " N4 f# s& A; @# @8 y. X
UNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta 2 G% g$ I  }) c" F
UNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells
. M) M  ]: P" C8 u8 UUNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
! Y1 U& t2 a) o- U3 ` Section F Mesodermal Lineages - o6 q: R' q/ f  ]) I
UNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
# q) {4 m) s9 ^& ]) m2 P3 e; ~4 ZUNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium
' c/ c8 J) h( g7 L9 X, `! N, IUNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells , a6 Q$ k5 ?7 b7 u, A
UNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood 0 E9 x" u  O' U. v& k5 k2 V) `' [+ v
UNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells ' U* j- p5 D) ], `
UNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
6 o. M0 L& L$ ?! V4 q: NUNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors
/ v. ^% S9 j$ c6 b# {8 EUNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells + V+ G$ w5 N4 Y- P9 N+ O
Section G Endodermal Lineages   N$ ^7 |# `* {$ P8 ~; c
UNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
# v4 h/ a# o. [# X; k' BUNIT 1G.2 Pancreas Differentiation of Mouse ES Cells
/ q  }1 P% S1 e7 W# z6 DUNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm & R  h1 Q% J, C4 s; Y9 ^! ~( E
Section H Ectodermal Lineages # b3 b: r; Z' D3 \! X" x" p$ m$ z
UNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
) I. J: d2 h: S; [/ d% ~. w0 R Chapter 2 Somatic Stem Cells
* x9 b* A" W% P+ k  C; l4 K Section A Hematopoietic Stem Cells . B2 j, f* W9 a# e1 A% `
Hematopoietic Stem Cells
) ?* u- d9 z$ h, NUNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient & m! Z: G: l6 P/ I- ~9 M: Z0 b! W# [
UNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
$ @5 E3 }* l. VUNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
" {5 Z  Y4 W. mUNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow ) f+ A& _% `2 U
UNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche
: N* O% L( B5 Y% V3 Z: q  hUNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues
$ n+ J7 b2 S3 G- KUNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
5 w( ^- C' h; L" `! vUNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells 5 L) g8 G# K' G1 I& R- V- _# C
Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells / G7 t/ h' s- Q9 G- V( I
UNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues 1 C7 V' i8 F# M" J- O6 E. P: t7 D
UNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells ' z# X5 E( h7 ]. j% o
UNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
) d2 q: t5 @) s) ?$ f4 K Section C Cardiovascular Stem Cells
8 ]! m5 w$ M. tUNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
' x, C$ c6 g; l1 Q7 `! tUNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium % A. h) b$ W+ ]: o
Section D Neural Stem Cells / [! m! L" ?0 `* L: _( q1 t/ i
UNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains 6 U  a- N: K! G# i# E) }
UNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells 7 w9 p1 e9 c8 q
UNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
+ M& o) @$ J% f+ m  T Chapter 3 Cancer Stem Cells
* ^4 K1 Q! L1 M, S8 BUNIT 3.1 Colon Cancer Stem Cells 8 W' N6 F0 i+ `, D8 ?
UNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model ; l" s  k0 k  r8 R  G
Chapter 4 Manipulation of Potency
" V0 X- x; [. ?: z7 v Section A iPS Cells $ S; `& V; F+ m) v
UNIT 4A.1 Human iPS Cell Derivation/Reprogramming
7 \/ _& p$ B% D8 E& JUNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells ; B% x# V" g9 t  f  _
Section B Nuclear Transfer / b1 w& k: Y& w- N8 i3 z2 i
UNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency
3 I0 y+ L( M8 F0 P* f Chapter 5 Genetic Manipulation of Stem Cells
0 Z) v' R3 P: m0 T4 w Section A Lineage Tracers in Stem Cells : o) X/ [% b9 c2 f
UNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
0 y: {! S0 `- _UNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing
% A0 ^0 ]# a' D0 ] Appendix 1 Useful Information
# y2 u0 O" u1 ]5 W$ e( h0 ?" q3 MAPPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research - D. U3 U; g. q( u8 [! a7 v
APPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
) K) X; s0 @3 ]: e/ X& i( j Appendix 2 Laboratory Equipment ! o3 R% Y! Z1 @" x4 ]0 p
APPENDIX 2 Standard Laboratory Equipment
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he_liang

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