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本帖最后由 细胞海洋 于 2009-8-13 12:01 编辑 " f0 g0 Q' R, E, L( y
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Current Protocols in Stem Cell Biology2 p8 w; O/ X0 ]7 ?
Last Update: July 07, 20095 X$ _' _9 H3 ~, A+ H
Print ISSN: 1941-7322
* C* G3 F# u& }" }$ K6 k# eOnline ISSN: 1938-8969
! t& s& E& B- p' m; x1 ^6 mTable of Contents
7 E* c+ S2 \% h5 H n: |Foreword & P# }& q4 _& \% ?5 G, r
Preface & F% a+ P" {; |' H3 y/ Y
Chapter 1 Embryonic and Extraembryonic Stem Cells
. e& B& b' l# l6 v( O/ C8 d9 J: X& l Section A Isolation of Embryonic Stem Cells " J/ I$ a4 U8 Y
Isolation of Embryonic Stem Cells
& \/ k4 l& _. A$ h3 T$ Y* lUNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells : t- u, q" Q% d _7 B
UNIT 1A.2 Derivation of hESC from Intact Blastocysts u1 M4 r/ z; u. u/ C: C
UNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells ; e% ~- o6 X" w0 E: w) `. A1 E& g+ e
UNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
) r$ l0 y5 W; ~: [! V Section B Characterization of Embryonic Stem Cells , y5 O2 X+ ~" v! E
UNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells % M+ m, l( b, T! _& x$ H
UNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform . k x" h+ P8 j: X( c6 _: W
UNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells # _3 |4 w# L) ?; y2 Y1 \
UNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
3 ?$ u1 V7 H7 y$ O2 i8 t+ }UNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
$ q# a% _ r) F; A" e# A: S7 T$ }# X Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
# w9 Y3 ~4 M, `( y8 g$ u$ {* |Culture and Maintenance of Undifferentiated Embryonic Stem Cells
% w3 x. f$ ~5 h. iUNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
; F- S5 r% i! OUNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture 7 M8 }2 v1 k+ |9 S
UNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells & Z* I' i) W: r! f
UNIT 1C.4 Culture of Mouse Embryonic Stem Cells
- K# y: e2 }) [, p/ xUNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells * t K. W) \1 ~9 |" |8 _& F% K7 P
UNIT 1C.6 Isolation of Human Placental Fibroblasts / X# n) C1 e( Z" Z* s
UNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
; a# f+ Y4 H% {UNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor , ]5 `6 r# ^) K( ~& V q' W
Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells - p, u' X1 f* A
UNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
* Q+ ~; P9 ~* g5 I. |5 IUNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
) r2 A/ ?3 E4 wUNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay $ \$ H7 t( X; i
UNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
. M) a" f9 M" F0 UUNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
9 I5 \# g# P" a# v8 V Section E Extraembryonic Lineages ( H9 Q8 e$ V% ?9 H6 Q
Isolation of Stem Cells from Extraembryonic Tissues
( C2 _/ Z! Z0 \6 J) nUNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells # F2 ^% G0 d I, e; I1 w, S7 H
UNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta ' c1 x* k8 }, v! t" r6 Z" }
UNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells 4 U+ S x# e, V2 Z% S# X, s/ e
UNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells ( p+ [+ c6 [) i9 F0 C
Section F Mesodermal Lineages
1 _4 d- D* R9 y6 e6 o3 bUNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
! h! a7 f. b/ q8 |6 k7 T% q$ d* }UNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium
- A( }5 F6 T) q$ [ N+ rUNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
8 f# U( U- n) {: zUNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
) g" e, p% c: r d4 q1 Z, a3 OUNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells 1 [; i2 B9 i/ [% @* {
UNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers ' Z U! i# Q, F! ?: h6 m/ r9 Y
UNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors X9 b" S: l8 F" @. p# e+ ?
UNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
$ M9 g2 F2 C; B# Q ?: a8 _ Section G Endodermal Lineages 1 A* D$ X. ], q; g Q
UNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells $ A7 @ l, Z6 p0 W2 ^1 ~! C
UNIT 1G.2 Pancreas Differentiation of Mouse ES Cells
/ L1 ~1 ?' M8 G7 Q: x( I1 ]UNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm 1 j. ]! | X1 S
Section H Ectodermal Lineages 1 ^1 a, w/ G7 u/ s* s0 Q
UNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons ; K: s5 M2 l: S8 U
Chapter 2 Somatic Stem Cells ! H: f6 ^: P0 ~* D/ v
Section A Hematopoietic Stem Cells
8 l) h) i7 q4 ZHematopoietic Stem Cells
0 N( q* J/ N2 k; \UNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient
- r( \6 | a9 v# MUNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood * a8 g! T, c n, a) |2 X& P5 {
UNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood + p6 Y$ P+ C: `; l8 a
UNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow / [2 K6 l0 D! X2 l' B
UNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche
' c0 q& r! r/ D$ M* N8 z: DUNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues . U- O. K: K( H3 U1 {" f
UNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells $ P5 ^, |0 H o3 D) W) Y9 @2 {
UNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
# @" ^, H- L# G: `8 v+ C Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells
. v# i+ B$ @3 X' HUNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
- T1 y7 G4 ]) ]9 oUNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
$ ^: N/ h. f5 E* W% i6 WUNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
' H& @; a' C5 w Section C Cardiovascular Stem Cells
% w4 w* P; @5 t6 t' M' xUNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood " e! n& L3 {# M2 w6 u6 z7 M: d$ w
UNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium 2 g/ E) P' D7 ]0 |
Section D Neural Stem Cells
3 z+ n _1 m% r3 [% W* GUNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains 4 s/ K; R* r/ Q L7 C
UNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
6 N4 Z- B8 d: W0 Z% J6 CUNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells 3 h# {: ~, ]7 O2 D D5 m* m
Chapter 3 Cancer Stem Cells & C8 C: y0 Z( x/ [1 x
UNIT 3.1 Colon Cancer Stem Cells 6 I$ {" f" s+ {, o/ J+ R
UNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
) p. B) H: t8 Y; T+ S) t Chapter 4 Manipulation of Potency
7 v+ k3 h$ W w# B8 @ Section A iPS Cells / C; }! u6 C% V5 Y% t* I4 c; F
UNIT 4A.1 Human iPS Cell Derivation/Reprogramming ( B0 M7 o6 ^6 f3 B
UNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
: m2 d3 x( }( |5 V Section B Nuclear Transfer
* \1 \$ k+ k1 i! PUNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency 7 G6 ?1 ^7 W) S# S
Chapter 5 Genetic Manipulation of Stem Cells
8 Q& E8 s s. }! k ^ Section A Lineage Tracers in Stem Cells . b j( x* f8 I) E a
UNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma 7 S% g( \1 ~* F7 S$ `1 e9 q! y
UNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing 7 n! ?+ y6 R8 K. K
Appendix 1 Useful Information " F/ C7 B w& P& K* u# w3 i
APPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
: {8 q- c# c G% VAPPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells 8 F$ }* n$ S1 l d+ H7 q+ z7 @+ {
Appendix 2 Laboratory Equipment
5 b' X- A& f7 bAPPENDIX 2 Standard Laboratory Equipment
! C( S- q1 I, }$ n% p2 H先谢谢啦！ |
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发表于 2009-7-28 16:42
发表于 2009-7-29 16:23
