[全文]丁盛Nat Methods新文章－－提高iPS诱导效率200倍

芷瞻

Nat Methods. 2009 Oct 18.【Epub ahead of print】
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A chemical platform for improved induction of human iPSCs.. |1 q( |  u* e6 a2 {

( I0 j) X$ K: t. LLin T, Ambasudhan R, Yuan X, Li W, Hilcove S, Abujarour R, Lin X, Hahm HS, Hao E, Hayek A, Ding S.
4 z1 l* ~  e% jDepartment of Chemistry, The Scripps Research Institute, La Jolla, California, USA.3 N# Z; V# o9 N0 @
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The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
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PMID: 1983816+ C8 ?+ _& E7 n8 _5 ]# R% F
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