[全文]丁盛Nat Methods新文章－－提高iPS诱导效率200倍

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Nat Methods. 2009 Oct 18.【Epub ahead of print】& w$ q* K# M0 F* O  X2 ?
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' N4 z( Y% D3 u' l: c3 yA chemical platform for improved induction of human iPSCs.
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Lin T, Ambasudhan R, Yuan X, Li W, Hilcove S, Abujarour R, Lin X, Hahm HS, Hao E, Hayek A, Ding S.
  D8 z" t' A. y1 ~* s$ uDepartment of Chemistry, The Scripps Research Institute, La Jolla, California, USA.$ G7 e9 ~2 {. g4 f4 ]+ {

5 j& ~* L/ w- E$ ]- U4 X- ?The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
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3 N9 Q# ]2 f8 D# K, ~9 l3 q$ a4 hPMID: 1983816
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