有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章* x/ s; P! t" x! y
2002 Lactate inhibits germ cell apoptosis in the human testis
+ s' |8 s' i( S  S% j# E2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture
' D' D( ^2 M5 I% R* k2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal: w* U) @3 C7 U5 G3 C- _3 d
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells* i2 \/ z5 o2 s0 M' g  P% p* |5 V9 H' J
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro' |" B% a3 y6 O% u1 Z' e% y, P
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture% o* x8 P% Y; l* f
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis- u- f( k7 x* }" ?. w- v
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture  F* L+ E! r) w3 Z6 C
2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse, A7 Y2 e! ~; v+ K$ `* L( r
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines
) `; I9 y/ t, c$ S( [4 @2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells1 n! x3 ?, H/ L1 {( X5 n7 k
2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells, j( D8 I+ b3 A8 R/ b
2005 Anchorage-independent growth of mouse male germline stem cells in vitro
! r0 g9 X9 ]- h+ A+ a2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor  ^) W7 l1 `! u; w8 C/ C
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat9 ], K- n  |& ?1 y( ?, B( F
2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment' \# S- P' A* d' M& f' l5 z
2005 Isolation of male germ-line stem cells; influence of GDNF
: ]4 ?7 g/ T& f. u2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
! q8 @4 R* E" s& Y1 _! l0 h  |2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture. N+ G- E4 c: ~9 n
2006 In vitro propagation of spermatogonial stem cells from KM mice. H+ U6 `" {! q1 m! c/ k' \1 Q
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上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的