有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章$ g% \* D' R( E1 |+ E* b. \
2002 Lactate inhibits germ cell apoptosis in the human testis# v2 ^2 v+ F5 H; a
2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture- T: D/ h) M( F" }  j* _- g$ r
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal9 V( F. F+ h* C
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells( t0 w. f+ a6 }- l
2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro  ~# s3 Z9 @+ ?# Z) }! b+ l
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture& T- e, |. A) R8 P/ J- e  C( y
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis
  c  ?; f* p" H9 n3 e6 O5 s9 G  E2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture+ T0 N. L( k( l4 n
2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse$ z  j2 ?  K  r2 X
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines
, |* L- R0 v6 d+ Q2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
7 S# z% b& S; \2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells
& D7 n6 U  x) ~  e0 p6 ?/ S4 c2005 Anchorage-independent growth of mouse male germline stem cells in vitro$ U  X& e4 H' Q$ z$ C$ G4 J
2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor
, R' E) J9 U& Y7 ~. V  R( l& G2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
8 Q4 H: D: M  g- s6 H# q) Q2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment6 x  J  s2 I. }& u' |
2005 Isolation of male germ-line stem cells; influence of GDNF# K  f  z; v) [; N$ L# Z% r! F; j3 x
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions) _7 n3 b8 Q% M% k
2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture1 @& F$ O/ c1 u7 p8 K% Q% c) V
2006 In vitro propagation of spermatogonial stem cells from KM mice
8 O- T5 w+ h3 ?8 F& ^! P, D
+ [7 J9 y+ R5 x% U- n上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的