有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章2 ?! L* E( r; F% G2 O# D3 W
2002 Lactate inhibits germ cell apoptosis in the human testis
5 C+ `- q* T4 a4 A, N2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture
- m$ K/ J! M- }. ^1 ^  M2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal
' [% h+ O$ \, m+ z- I2 H: \0 \; R8 X7 g2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
9 ?9 c" G" Q! l; r) u) L! |1 O2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro3 c7 E7 I: w9 F' x# n5 D7 s
2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture+ o$ A- f9 Y6 o3 T# z; `, c
2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis
, S, }7 `% ~* M% r2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture. ~& _/ o9 L/ h8 K
2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse
6 T( ~: v  x; ], W% D# `' C2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines% {" F9 ]$ j, u0 `: H$ ~% E: a: W& T
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells+ b$ k4 G( g. ^3 P7 _+ ~3 E
2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells0 a3 \3 X& Z, ?
2005 Anchorage-independent growth of mouse male germline stem cells in vitro
$ Z3 U! [$ u  I  D2 Q5 {6 {5 c2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor
3 O: S6 E! I$ i* f2 A2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat: [3 c% g6 s) \4 a& T6 L' O
2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
1 h7 _5 W9 C; ~6 ^2005 Isolation of male germ-line stem cells; influence of GDNF  x+ V3 W" {5 j- ]: O% u% a
2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
  G7 o* G2 f0 @$ W' p2 ?2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture
5 p* P. S: M4 T  F$ }" K2006 In vitro propagation of spermatogonial stem cells from KM mice
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2 \( i9 f9 o1 u6 B' V# ^! h3 M上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的