有关精原干细胞培养的文章 补充中

ellapan

欢迎加入讨论,如果你接触精原干细胞,即出生后具有self-renew功能的生殖细胞,与前期一直proliferation的PGC不同,它不进行大规模的有丝分裂,而是进行所谓的不对称分裂活动(当然这是在体内in vivo的情况下),在体外培养同样能进行扩增,虽然能传代,但目前并没有建系的报道.关于现成的protocol,我推荐你看humana press的germ cell protocol中的Volume I的Chapter 8:spermatogonial stem cell.上面给出了详细的分离,培养过程.但是我觉得并不十分完美,所以我推荐你看下面的文章
; p( t  f# r7 E& Y+ v; F2002 Lactate inhibits germ cell apoptosis in the human testis
3 g  \' l$ M+ d, v& t* O2 t2002 Maintenance of adult mouse type A spermatogonia in vitro influence of serum and growth factors and comparison with prepubertal spermatogonial cell culture% K% E. j3 ]7 u% j. S+ W
2003 Clonogenic Culture of Normal Spermatogonia In Vitro Regulation of Postnatal* l5 c/ e- b6 Q
2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
/ C: z9 X9 j( x4 M( ?, Q+ O7 P1 h2003 Maintenance of Mouse Male Germ Line Stem Cells In Vitro
! d/ |* @. [) Z# k0 [2003 Proliferation and Differentiation of Bovine Type A Spermatogonia During Long Term Culture
4 C% H1 Y! J8 |6 h$ r$ v2003 Proliferation and Differentiation of Spermatogonial Stem Cells in the wwv Mutant Mouse Testis- i  ~3 Y' ^9 P# Z$ o
2004 Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture
$ b1 R/ H: y) d* w+ m8 |2004 Culture Conditions and Single Growth Factors Affect Fate Determination of Mouse  H/ f0 J9 Z1 z" N) g( f
2004 Derivation and morphological characterization of mouse spermatogonial stem cell lines& @9 ~' B; Z1 X  _; j+ t
2004 Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells
% S- Z4 g# O7 K9 ?9 Z6 l5 |5 ]# V2004 Testis Tissue Explant Culture Supports Survival and Proliferation of Bovine Spermatogonial Stem Cells
: b! B& U/ F# Z8 w1 P2 d2005 Anchorage-independent growth of mouse male germline stem cells in vitro! |! Z- r8 y: w. V3 B
2005 Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor, F; w8 O6 [. x2 g1 q
2005 Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat
  S! \: t' L& o; h2 H  X# Z" k2005 Efforts to create an artificial testis culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment4 _. f/ s- f5 o  c6 I& t) W6 i
2005 Isolation of male germ-line stem cells; influence of GDNF
2 ^+ b) ]3 A3 Z0 C2005 Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions
$ u1 p' ^7 U& v! c; Z2005 Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture5 g" F+ @# K6 r/ e
2006 In vitro propagation of spermatogonial stem cells from KM mice' J1 G% d0 U. X; w9 w7 T
. y& c6 c0 g4 U. V. [
上面的文章都是成功培养的例子,但是精原干细胞的培养是相当昂贵和耗时的