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We performed three additional experiments using CD2715 M" M2 K7 x; G
for enrichment of MSC. Upon isolation of MNC by Ficoll2 g6 i" s8 @: D9 {7 W
density fractionation, half of the sample was processed
( u% E* `# L9 n: h( Z1 i6 Zfurther using the CD271 MicroBead Kit. About 5% of the cells were in the positive fraction and about 50% of these4 z9 ]6 v+ }% ]& Z; s6 }& g
were CD271
, as determined by flow cytometry. CFU-F4 B+ l) w5 ?% _+ d1 y; ]
were exclusively found in the CD271
fraction, as- f/ R6 E0 \7 w, |+ s# u6 S
described elsewhere [22]. The number of CFU-F per" F9 ]. S9 j+ ?- T2 X: k4 K
cell was 210 times higher in CD271
cells in comparison
' k( E4 T2 o2 b+ H5 {1 g( `with MNC. Hence MSC were enriched in the CD271
/ C+ V1 f" t: h! H- l
fraction, although the yield of CFU-F per milliliter BM
# [) f+ n- k7 R$ c0 `5 Kaspirate or with regard to the number of MNC was greatly
. Z; f% B% h! \0 S0 Sreduced.

oldmac7

我来试试看:
5 x! U! s+ Z8 g5 E
& a" t, ]( k* D. x! @* i7 M3 ?. v我们进行了三次另外的的试验, 使用CD271来丰富间充质干细胞。经用Ficoll密度分馏来隔离间充质干细胞后，对当中一半的样本使用CD271微珠子包 (MicroBead Kit) 来作进一步处理。约5％的细胞是在阳性的部分，而经流式细胞仪检测后, 当中大约有50％是CD271。 CFU – F往往是被发现只存在CD271的部分里，正如在其他地方所描述一样[22]。与CD271细胞在间充质干细胞的数量来比, 每细胞里的CFU-F数量 是比它高210倍的。因此，虽然每毫升骨髓抽出(BM aspirate) 或间充质干细胞的CFU-F产出量大大减少了，间充质干细胞在CD271 的部分是被丰富了的。