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We performed three additional experiments using CD271
8 i( u$ D1 Y: C( Xfor enrichment of MSC. Upon isolation of MNC by Ficoll
' @7 N3 b. q5 O3 j' y2 k/ jdensity fractionation, half of the sample was processed0 v. s* d% w" D. O" J# l
further using the CD271 MicroBead Kit. About 5% of the cells were in the positive fraction and about 50% of these1 M& N" T& I0 F* ?& L" ?
were CD271, as determined by flow cytometry. CFU-F" C1 U; |9 ^' f
were exclusively found in the CD271 fraction, as
1 W* i, H% x- e7 j- _+ Z7 \0 s! O7 Rdescribed elsewhere [22]. The number of CFU-F per
- O+ [$ g% I/ K. f' A. h$ bcell was 210 times higher in CD271 cells in comparison1 Z8 J6 D& r1 J7 a Z
with MNC. Hence MSC were enriched in the CD271
. j7 \2 I4 M7 E3 e, Cfraction, although the yield of CFU-F per milliliter BM4 P- f% T* N7 V0 h: Z7 J$ e
aspirate or with regard to the number of MNC was greatly+ d1 {/ a" u8 _- G& P% p
reduced. |
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