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本帖最后由 细胞海洋 于 2010-4-19 11:09 编辑
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Cell culture is an invaluable tool for investigators in numerous fields. It facilitates) ~' `* T' `; d. z* P
analysis of biological properties and processes that are not readily accessible at the level
; s" K e5 v9 Z3 K F: zof the intact organism. Successful maintenance of cells in culture, whether primary or H2 F8 U( M/ e
immortalized, requires knowledge and practice of a few essential techniques. The purpose
) u3 i: t) E1 Uof this chapter is to explain the basic principles of cell culture using the maintenance0 W4 [+ C4 m4 x6 ?3 Y8 {
of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation
/ U+ k0 T: y6 X e# B8 jand culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples.. g( y( ^& [; ]2 s) K/ f
Procedures for thawing, culture, determination of cell numbers and viability, and
6 Q& }9 J. L }% V1 B% bcryopreservation are described. |
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发表于 2010-4-19 10:40
发表于 2010-5-25 10:08
