请教piggyBac transposons的缺点。xx

dispassion

从原文上看，piggyBac transposons是不整合的可以移除的。可是Junying Yu对它的评价是: S* {, C9 P. r3 G
A second approach used seamless excision of
; h8 \7 N  U/ b* d! L% I8 ~6 K5 N0 hpiggyBac transposons to produce vector and transgene-free
1 h/ @: ~' |% a' a5 zmouse iPS cells (10). Although a promising approach, vector1 S( r: E% _( K9 f8 A; @( s
removal from human iPS cells produced by this method has
$ ~8 {9 {- o0 {7 e+ u* jnot yet been reported, and removing multiple transposons is
" w# b: Y$ L- L1 I0 [labor intensive.5 ?# P2 P' G5 x1 o8 B' \
我想请问她说的缺点是从何而来。谢谢