干细胞移植治疗实验犬心肌梗死后心力衰竭的作用

海人

干细胞移植治疗实验犬心肌梗死后心力衰竭的作用.pdf
$ f: `6 c8 v3 p% ]& \- v徐赤裔周建庆方建江廉孝芳) M, E! q6 A  Q' W$ z+ Y
【摘要】目的初步探讨干细胞移植对犬心肌梗死后心功能的作用。方法取犬脂肪间充质干细胞(ADM—
  G  E$ ~7 p6 w' x0 d! iSCs)进行体外分离培养和4 ，6二脒基一2一苯吲哚盐酸(DAPI)标记，将犬随机分为2组，对照组和ADMSCs移植7 {8 [% l' J* I5 d  A: t
组；于术后4周行超声心动图和衄流动力学检查；并处死动物取心脏标本，分别行免疫荧光化学观察移植细胞存4 M+ j5 v0 }+ B9 C0 y
活、分化情况及免疫组织化学心肌特异性肌钙蛋白T(cTnT)检测、测定血管密度和胶原容积分数(CVF)，计数凋亡
# s) z( h7 m4 q2 [/ {细胞数。结果犬脂肪组织中含有大量长梭形细胞；术后4周DAPI阳性的ADMSCs可见于梗死边缘区及梗死区，+ b0 H" ?6 ?/ L9 {" I# }
且分化为心肌细胞表面标志物cTnT阳性的细胞；ADMSCs移植组左室重量指数和左室收缩末容积(LVESV)、非梗
0 x, c6 e+ p, V9 _) L. p死区CVF和凋亡细胞数均减少，左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室等容收缩期室内压最大上4 M) L8 n5 y* x* b
升速率(+dp／dtmax)、血管密度和梗死区CVF均增加。结论 同种异体ADMSCs移植后，移植细胞通过分化为心肌
  p. x( R' V. `7 M8 W1 }' S样细胞，促进新生血管．抑制细胞凋亡，减少非梗死区胶原沉积，改善心肌梗死后的心功能。% N) g* v1 ^% o3 W( }
【关键词】干细胞移植心肌梗死心力衰竭
( Z# ?- O, ?5 C8 P% J0 m' b) p% OThe Study of Stem Cells Transplantation for Treatment of post- Infarction Congestive Heart Failure XU
! [6 ^1 n5 c' @- W- miyi ZHOU Jianqing,FA NG Jianjiang,et a1．Department of Orthop~dics．The Second Affilimed Hospital Zheiiang U—
' I( b* X4 R  Zniversity College of Medicine，Hcmgzhou 310009,China
) d) M+ R* a, S) ^【Abstract】Objective To explore the efect of stem cells transplantation on heart function'after myocardial infare—
0 W: Y( E4 s5 ?+ h8 Mtion．Methods The abdominal hypodermic adipose tissue of canines was isolated，culture in vitro and marked by DAP1．Ca—8 F$ ^; P- C) q& |7 v  ^( [
nines were randomly divided into tOW groups，control group and ADMSCs implantation group，which received 1MDM medinm: \& m  ]; h; S" |8 Q. u) K" Q
and ADMSCs which marked by DAPI．To test heart function ehange．UCG and haemodynamics were measured in each group
7 `3 i! ^" W4 K4 A$ Y+ @3 Aat 4 week．4 weeks later immunofluorescenee was given ohsmwe the information of the transplanted eells survival and differ—
9 a  ]: `4 A; b, J, _/ rentiation．After M1 and cell transplantation for 4 weeks HE staining，immunohistoehemieal，capillary densities，CVF and. p1 z# t& K* X$ D7 L4 B* H
apoptosis cell population studie．Results There was a large amount of mesenchymal stem cells in canines adipose tisslle．& `' Y+ y$ ^9 C" P
Smwiving ADMSCs were identified by DAPI positive spots in border of infarcted region and transdifferentiated into cardiomy——
$ r" N9 l! X8 y& W* w' Foeytes characterized with a positive cardiac phenotype ftroponinT)4 weeks er transplantation．LVESV、left ventricle ponderal
& i& [. u9 Z0 z( tindex、CVF in noninfaretion zone and apoptosis cell population of ADMSCs transplantation group were lower than con—
  `  e9 G* r  j( b/ a- ctrol group，whereas LVEF、IXSP and+dp／dtmax、capillary densities and CVF in infarction zone were significantly higher．* R1 f( t8 g( X! {$ w' u. r% W: k
Conclusion ADMSCs transplantation can imprmre l heart function after myocardial infarction in canines and the possible
: j: [1 p1 }: K- Yreason may be correlate with mieroenvironment and produce a marked effect by angiogenesis，inhibition apoptosis and redue—* ~( {& V- Q! B0 Z, r% q
tion collogen deposition in noninfraetion zone．
. V8 c& [: b4 z& ]【Key words] Stem cells Myocardial infarction Heart failure$ \' f: Y: V) P
[hide][/hide]

月飘东海

我要看看嘿嘿

siwa

很好，参考一下，谢谢

ligangcangxb

可以参考

在路上

1# 海人 [/b谢谢

luohaijian

哥吃的不是面

天空为我蓝

回复 1# 海人 ) c! t. W) H7 {+ X( G: X
0 B* t& O% \5 A9 N: m: T# {. R+ P
( |# ?. r5 |, G
    谢谢,看看

桂圆儿

谢谢

study

想看看，谢啦