knockout DMEM是什么意思，knockout血清替代品呢

znhy

knockoutDMEM的具体成份是什么啊，它敲除了什么？还有血清替代品具体成份是什么？谢谢

daviehe

knockout是一个品牌，knockout DMEM是一个基本培养基
) {0 A4 f7 b( j8 Nksr是一种成分明确的无血清配方，可直接替代现行方案中的胎牛血清
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具体如下9 R! }& H, F5 o/ ~9 a% p
1。KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells . The osmolarity is optimized to approximate that of mouse embryonic tissue.- ]: D" s9 V# A! X6 z) Q
2 Knockout-SR is a bovine sreum albumin(BSA) solution with lipids,vitamins,trace elements, and low concentration of transferrin and insulin.' ^" ]" m, w  I$ |! F: _
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KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures.  ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least.  The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture

znhy

回复 2# daviehe
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    哦，这样啊，真的十分感谢！！

daviehe

不谢

lifescience1

这个可以帮助你理解Knockout DMEM 哈

ambassador

knockout是一个品牌，knockout DMEM是一个基本培养基
8 X9 Q" P. K! E% f2 W1 cksr是一种成分明确的无血清配方，可直接替代现行方案 ...
2 \3 p8 }: D  M# J% o0 f/ L) X% Mdaviehe 发表于 2010-5-14 16:24

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! L. w& `% e/ p- G( o% K4 A   谢谢解答！

znhy

回复 5# lifescience1 9 y3 D# r, G$ s$ q* Z. b3 d

0 l5 N* F, k! w6 ~0 I很好，谢谢了！

afei198520

学习了 呵呵

leestone

Knockout™ Serum Replacement (KSR) 雖然成份明確: v# B8 |% F4 B& B* r+ ?, S
但是Invitrogen 基於專利保護的理由並沒有公開詳細的成份
; E9 ?# J6 x5 {只提到專利申請中(可能怕使用者自己調配吧）
2 j" [- S; |) @4 P' S& H也就是說) R, W' }- G9 V2 h: }8 x3 o/ ~4 Z
成份明確，但是不讓你知道....

简简单单

好，学习了