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本帖最后由 细胞海洋 于 2010-6-19 13:37 编辑 3 [9 m* ]* E; D) F3 y
8 Z- o3 V7 l. x# P9 {FOREWORD
, @) Q, Z m! v2 G- JStem cell biology is emerging as a field in biology with tremendous therapeutic potential.# j3 L1 M3 m! `" \4 Z( }' n4 g+ y0 N
Making this potential a reality requires an international effort. The recognition
/ c! h- u# E/ Y5 }that such a promising yet multifaceted discipline needs fostering led to the establishment: [# S8 C& T! b. ]9 f: ` C
of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the) |0 y7 R# s0 T' F. |$ X" y
ISSCR is the development of tools to ensure the success of stem cell researchers. What
/ R$ \4 {" @4 H( ^4 D8 Kbetter way to do this than to collaborate with Current Protocols to develop this valuable' q" D; |9 D1 U% d* L- O- o8 d' `5 M
compendium of protocols in stem cell biology?
( _" s' |; t. b) b7 [Stem cell researchers have developed a number of breakthrough techniques, from the
& d' z8 @' u }derivation and manipulation of pluripotent embryonic stem cells to purification and r/ ~ {/ X% f, ] A
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories% H+ C* R7 L$ U
have become leaders in the field as a result of developing such techniques. The more
: e2 @* |" o' r$ a9 E3 q/ Wefficient scientists are at implementing new and powerful methodologies in their own
, q7 {9 m) |9 o* e9 x& ^laboratories, the faster stem cell biology will advance our understanding of normal0 ~% y9 T2 b( ~* s4 @ K8 D; o
development and lead to the development of therapies. Thus, the availability of quality2 z' |9 T0 f' `
protocols will have amajor impact on the success of the entire field. Current Protocols has$ t% h7 \2 W$ _2 B: z
long been the premier volume for proven in-depth protocols regarding many aspects of }9 d1 ]' i4 T; m
biology, and this volume on stem cell biology will prove a valuable addition to researchers
% X6 `9 x/ U Rworldwide.
9 Z; f3 Z3 B3 l2 U9 SExperiments in stem cell biology must be interpreted with great caution as well as
* R# s; k# K3 b: J9 u( V# ~openness to alternative explanations. For example, the recently discovered phenomenon) A: P- t. ?$ a( {( \
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral2 m6 }6 D6 K0 L0 U* K
tissues were initially misinterpreted as evidence for stem cell trans-differentiation.' m/ G! K* M7 f% \9 d
It is very important that this compendium of protocols highlight potential pitfalls as well' B; ]: O: K( z2 ^9 q- g$ l7 r
as maintain the opportunity for clarification and correction when the need arises. The
/ N9 w" d& i; w" Q( }fact that these protocols will be provided online will help ensure that researchers always
- V$ p8 y! T7 ~3 I$ |+ ]- zhave the latest, most up-to-date protocols available to them.8 U( E: z& x8 p5 j. m5 {; O
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine
# t) b; c4 |% d g! X" K' L$ rpush to share information and interact so that the field can move forward quickly. There' l! M1 w7 @7 e# k+ @
is a drive to develop not only excellent basic research skills but to bring the findings7 R1 y1 V# l3 o5 f0 p3 n: o/ E1 s
to clinical use so that patients can benefit. As a Hematology Attending Physician at2 i5 B7 }2 t% F4 v2 ~" b
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia
5 z8 Z4 z) I$ J4 m# z, M7 mand am drawn by the need to translate our research findings into therapies to help treat a
V _& X2 G. h) k- S; wnumber of diseases. The promise is great, but we need to deliver, and I believe Current
( [1 ^3 O9 Z: @, |) zProtocols in Stem Cell Biology will help tremendously. I- {3 R: U. F' P! f" {4 i
Leonard I. Zon
4 P' G# k4 t- e$ n. @7 M5 e( j% nCurrent Protocols in Stem Cell Biology: C& R/ C# E( H M: ?. f6 u
Published online June 2007 in Wiley Interscience (www.interscience.wiley.com).* M B6 u8 K8 Q8 x6 W
DOI: 10.1002/9780470151808.scfores1( W0 R$ k) {! w# I/ I F/ T
Copyright C 2007 John+ q; i) @6 a# i$ [
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