肝细胞与骨髓间质干细胞共同培养时的肝细胞功能

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黄艳欣, 杜雅菊, 李宝杰
3 m3 \" }! h3 h" l3 l3 F$ j黄艳欣, 杜雅菊, 李宝杰, 哈尔滨医科大学附属第二医院消化内科 黑0 e% a7 w9 R& T  s1 p# A6 C
龙江省哈尔滨市 150086
7 p+ Y8 H3 N" l$ J/ S3 }黄艳欣, 女, 1977-11-14 生, 黑龙江省哈尔滨人, 汉族. 2001 年哈尔滨医科
; C2 T7 g9 _# r; Q, G2 ^% S大学硕士生.
& |' c' o8 [. Q项目负责人: 杜雅菊, 150086, 黑龙江省哈尔滨市, 哈尔滨医科大学附属第
' P8 Y. J8 i6 }, c/ b二医院消化内科.  K" P0 s6 T, b; V( d
电话: 0451-866051432 E4 V/ h* m3 E  Q2 ^; a
收稿日期: 2003-12-10 接受日期: 2004-02-01& j, r5 P4 d0 X3 s/ O
Effect on function of rat hepatocytes
' E5 L2 b7 `/ Fcultured with bone marrow cells' R# g- o2 q# b( H3 E7 T; w# G
Yan-Xin Huang, Ya-Ju Du, Bao-Jie Li4 G0 {! C9 A; [% Y
Yan-Xin Huang, Ya-Ju Du, Bao-Jie Li, Department of Gastroenterology,1 E  p* j: }3 p2 K8 `- \6 ]* [% }, N5 @
The Second Hospital of Harbin Medical University, Harbin 150086,9 v- L2 y, I) O$ Z
Heilongjiang Province, China3 n, x4 w. B8 X6 J1 ^
Correspondence to: Dr. Ya-Ju Du, Department of Gastroenterology,
/ i9 `8 a' [% {6 k' W/ n1 LThe Second Hospital of Harbin Medical University, Harbin 150086,5 v: t! F% q) F) R4 b( t
Heilongjiang Province, China
; ~$ [6 w% j8 I& O# @' }Received: 2003-12-10 Accepted: 2004-02-012 l! }7 O, h/ ?3 _8 h
Abstract
/ ?$ U: v1 x; T' m# c; m# x+ Z+ }AIM: To study of the effect on the function of rat hepatocytes
+ d/ q* U9 z+ P3 pcultured with bone marrow cells.8 c9 q/ I7 ^+ Y, L+ D
METHODS: Rat hepatocytes were isolated by the modified
6 U! D9 r0 @# X9 ftwo-step method described by Seglen. The primary cultured
8 R) i* L4 o1 D4 C9 ghepatocytes and bone marrow cells were served as cocultured. y7 p* X( ^, T( V6 }, v2 x
group, and single cultured hepatocytes as control group. The6 v$ R! t# e+ E2 p$ a; D0 p8 N
yield and viability were assessed by trypan blue exclusion.
! W- y: Y- D; D8 t8 P  W* _The morphologic changes of cultured hepatocytes were) H: y- o, ]6 d
observed. The concentrations of albumin and urea in the2 V2 m( z6 u& A3 z2 \  K5 D
supernatant in different cultural period were examined.$ W9 d+ O* h6 m* `8 O2 s
RESULTS: The albumin synthesis (13.75>2.179, P <0.05)
2 T- t6 f& B! F! f. [- e6 f5 Land urea level (7.27>2.179, P <0.05) had fluctuating
0 ~/ [' l+ f4 r& \6 @, m  rchanges in one week, and cocultured group had higher3 k/ q& e# ]: d- U; E( V
albumin synthesis and urea level.& y3 V8 J6 B6 z; M; }; ~
CONCLUSION: Cocultured hepatocytes with bone marrow
7 _# ^  h0 \) r3 i5 C0 w# o7 acells can improve the function of hepatocytes.
1 ^* U" q' p5 W6 _8 _* RHuang YX, Du YJ, Li BJ. Effect on function of rat hepatocytes cultured with: u% p9 z& N5 F' O! |1 \) J
bone marrow cells. Shijie Huaren Xiaohua Zazhi 2004;12(5):1129-1131& C: u6 R% s  q! k* ~& G
摘要
+ A6 Y4 [6 P% a9 O; e) d$ v- n: F目的: 利用原代培养的大鼠肝细胞与骨髓间质干细胞共同培
8 [2 _) e6 q; \+ r4 {养研究骨髓间质干细胞对肝细胞功能的影响以便更好
! B1 F, V1 v' D7 f1 v4 B- ^+ ?用于肝细胞移植及生物人工肝.
: q/ y9 a' ^% |( t: b' q方法: 采用低浓度胶原酶原位循环灌流法分离大鼠肝细胞
. _; `0 n) o' \8 o+ |. J5 x获得有活性的肝细胞进行原代培养. 台盼蓝排斥法计算细胞
7 N8 F  i9 u9 J产量和细胞活性; 光镜下动态观察细胞形态学改变并收集不同时期培养上清检测其细胞分泌等功能. 比较肝细胞单6 _% l( {1 B* O2 R' B4 V! W" }
纯培养及其与骨髓间质干细胞共同培养时细胞的功能.
5 I( Y/ U* Y( l8 y8 ?& m0 o' ~. h结果: 培养7 d时两组均仍然维持白蛋白分泌及尿素合成功
' e- {- y  w- k5 Q能; 共同培养组与单纯肝细胞培养组在白蛋白分泌 (13.75>1 N% t; t! y1 Y+ U& d& Y  D$ \
2.179, P <0.05) 及尿素合成功能 (7.27>2.179, P <0.05) 存在
) T/ k6 j8 \& `% r' ~+ n, t1 i显著性差异共同培养组明显高于单纯培养组.. l: V, Y- y; P" {% v2 h
结论: 肝细胞与骨髓间质干细胞共同培养可以使肝细胞
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franklinhg

谢谢  共享

uuyuq

学习

baichunyu

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支持

碧海蓝天

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yubingzheng

谢谢楼主

cncpht

good

tjutiger

baiduyixia

yuheng

MSC分泌因子支持肝细胞生长，可是比较怀疑单纯共培养是否能有那么好的效果？

missyoudad

好文章