|
 
- 积分
- 460
- 威望
- 460
- 包包
- 18
|
本帖最后由 FYH 于 2010-7-10 00:16 编辑 & e3 O7 _% |$ N# e6 Q, }
* s# O, {8 ~$ s2 j人ES细胞自研究以来，其培养的复杂性是制约其应用的一大方面，先前的培养中，需要铺滋养层或动物来源的基质胶。由于动物细胞与成份的参与带来免疫原性，而且加大ES细胞的自我更新与干性维持机制研究的复杂性，应用的安全性和有效性更加被置疑。而最近的三项研究表明，人ES细胞可以在特定的基质上长期培养，这些研究成果报道在最近同期的nature biotechnology，[Letters, p. 606, 611; BriefCommunications, p. 581; News and Views, p. 562]这为建立ES细胞有效的培养方法迈出坚实一步，在未来的药物筛选与评价，疾病模型的建立与机制研究，甚至是基于其的细胞治疗都有重大意义。（同时附上CELL STEM CELL对此研究的评述。希望对做人ES细胞与iPS细胞的同行有所帮助。
) t+ A9 U* n( k3 O
. `4 p, B& ~; d- {0 @$ K7 |. K
4 H- K7 Q) g0 j8 T, JDefining hESC culture
V* n: F( j9 v: q9 V. t. s; t MHuman embryonic
0 N* B8 ]5 T2 u! Y/ Qstem cells (hESCs) in
+ W* s r: h0 U6 nculture—at least in the0 O7 K5 {8 M# [* d o
culture conditions we have/ r; y& d; e% l
today—tend to lose their
% e6 d% C# d0 G( e6 @# O3 ^; idefining characteristic
o( A6 Z: {1 L3 w: N* Q1 m5 aof pluripotency. To
1 o; k* E: |# R5 j+ K" lmaintain them in an( ]5 m; j2 S, }' r
undifferentiated state,
# \1 a1 a2 G5 _+ b; B( Q8 |: Sresearchers often rely: i5 n- O9 O* }- h; V. R& c& b" ^ f
on complex culture5 y3 F: X' ^( d" ^! |+ t4 B
components such as& N4 L. g: P7 C* o W" n3 @, c
mouse ‘feeder’ cells
$ o, E% _6 u! @+ \1 I& Uand Matrigel, an extracellular matrix–like substance derived
+ {( b/ G3 V" }$ O/ m6 l; Ufrom mouse sarcoma cells. But these materials are xenogeneic,$ n* L. g y: _
chemically undefined and variable from lot to lot, compromising% Q( Y/ J- k$ o6 K+ K
the reproducibility of experimental results and raising safety; e- w4 p9 E* l3 B8 L( H
concerns over possible contaminants. Feeder cells can already be; K9 s1 |; v5 u1 f2 V% ]5 b/ @0 G
replaced with fully defined media, but a satisfactory alternative to
: n6 D- o8 X8 C) q: u* [Matrigel remains to be found. Three groups now report fully defined
- h: V0 m& @( T! ~' _substrates that support long-term culture of hESCs. Melkoumian
) U A2 \& j0 c* Nand colleagues show that peptide-acrylate surfaces bearing+ ?5 O1 K: \, _7 w, `
synthetic peptides derived from bone sialoprotein and vitronectin- x. ~$ ? r# l
are suitable both for maintaining hESCs and for differentiating; l$ e8 q7 p0 A+ R& ^" M
them to cardiomyocytes. Tryggvason and colleagues use a+ m( s4 N0 s- F& [% B0 ?6 E: @5 h
recombinant form of laminin-511 to culture hESCs and human+ m& O: |8 p* n- ^) A8 @. w/ A
induced pluripotent stem cells. Finally, Smith and colleagues) ?# B: r( `1 F* T' R9 f6 Y
culture hESCs on a surface made of the synthetic polymer poly[2-
0 W6 W- J7 p/ f- o# u- j- k(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium5 q6 ?, O5 @' r' I$ P3 U
hydroxide]. In combination with fully defined media, fully defined+ @! X( i% U) ?7 m# R
substrates should improve reproducibility and safety as well as
( e- z: [$ j8 t& r3 ?+ f4 yfacilitate scale-up of hESC production. [Letters, p. 606, 611; Brief3 r! X0 l8 V% q; J
Communications, p. 581; News and Views, p. 562] |
附件: 你需要登录才可以下载或查看附件。没有帐号？注册
-
总评分: 威望 + 20
包包 + 30
查看全部评分
|
发表于 2010-7-10 00:12
发表于 2010-7-11 18:41
