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- 积分
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- 威望
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- 697
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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml.
- H1 `! ]4 \" b# V c# S+ ] Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells
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Caution：ECM gel may be stored up to 72 hours at 2-8 °C.
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4 D- f8 p9 v& N, q. d rOther Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.
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5 Z* A( g, W- s/ B1 V1 y# @Properties* F" _% l- z! }
sterility dialyzed against chloroform
9 ^' A* W9 B+ Z3 E* Bform liquid
% `3 [8 Z' X: f. W1 f) t" M: f0 {1 g* Sconcentration 8 - 12 mg/mL
" J, X. G1 }! ^: Psurface coverage 6‑10 μg/cm2
4 |8 B& S# D; ~% b5 jtotal impurities endotoxin, tested
z j h* n+ D2 i0 gsuitability cell culture tested
: D5 J" @& y! d) c9 X) h- m1 @shipped in dry ice / V) b% v2 h- D% l+ d! C
storage temp. −20°C |
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总评分: 威望 + 5
包包 + 10
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发表于 2010-8-11 10:46
