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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml. ) _4 d8 o" H4 e2 }0 v
Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells 4 @$ D/ I G. [
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Caution：ECM gel may be stored up to 72 hours at 2-8 °C.
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Other Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.- e4 _- P' W# k& E: \
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Properties/ E' U% X9 u9 n3 P4 f+ C2 S
sterility dialyzed against chloroform ( ?8 M" z; H* R
form liquid # | c4 ]8 N' f4 T8 L$ H
concentration 8 - 12 mg/mL 5 b2 ]% x g+ H
surface coverage 6‑10 μg/cm2
5 \ d6 Y; `1 a: ]8 u8 ~8 btotal impurities endotoxin, tested , v! J/ P; A* [
suitability cell culture tested
0 o" p5 |' e; Z) q# @4 Pshipped in dry ice
0 P" d) k3 X: Lstorage temp. −20°C |
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总评分: 威望 + 5
包包 + 10
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发表于 2010-8-11 10:46
