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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml.
* M2 l9 A. [, |* Z* W Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells ) J% w6 k+ h8 p
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Caution：ECM gel may be stored up to 72 hours at 2-8 °C.
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# r7 P; @8 [ BOther Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.
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Properties
" Q w5 `; M/ ^sterility dialyzed against chloroform
# M% |% N2 j& j" M, ~8 E- w& Dform liquid
- x6 w* U8 m8 \9 I) P9 {. @concentration 8 - 12 mg/mL
1 \4 y: ?( b5 U6 Rsurface coverage 6‑10 μg/cm2
# H' v! R+ C. v* [7 E+ k6 W, wtotal impurities endotoxin, tested
4 [. S! e! \8 b v) D- ksuitability cell culture tested
! a" r; \: W: K6 ushipped in dry ice
- G; q2 l! {/ o8 O3 Y% D. }+ n4 R/ wstorage temp. −20°C |
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总评分: 威望 + 5
包包 + 10
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发表于 2010-8-11 10:46
