PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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0 u  C5 h" W9 u, r( ~. T4 S+ G. BCurrent Protocols in Stem Cell Biology 2007年完整版 1451页0 ^: C4 Y2 c( Y# J3 y) B( P

$ }- c/ k0 e' ]Online ISBN:9780470151808. a/ Z9 Y* B  c5 f1 S1 ?" @  Z$ W
DOI: 10.1002/9780470151808' _! O* L  V# Y7 Z+ {& G
Table of Contents& O0 \& q# v+ M, b$ o
1. Foreword
  R: O- {: {8 Y* W2. Preface
& H$ n8 ?3 X+ y9 b. Q. K, P3. Chapter 1 Embryonic and Extraembryonic Stem Cells: n0 ~+ k$ y  `  e/ M0 l
1. Section A Isolation of Embryonic Stem Cells
, }- |8 d: \/ m' H1. Introduction
) r2 A$ N# J$ m1 V; H( J2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
( ]8 p/ P  [6 s3. Unit 1A.2 Derivation of hESC from Intact Blastocysts6 [5 H0 g$ H9 F5 u. R' h, P! r
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
4 c" b9 q7 Z* N5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
' Z6 k8 w2 g% I6 C2. Section B Characterization of Embryonic Stem Cells* {) g; ]2 `: j5 W
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
; D# t' o; ~0 e) T2 n- b# O# F! x2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
8 V% n* N* }* X9 FEmbryoid Body–Derived Cells Using a High-Throughput Microarray Platform- d# x# {- ]. X& z! X4 A& P
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells, W: n# t% q& |; I) {' n
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of
/ T: a) j) [% A/ gPluripotency0 q  O6 N# j, P: F
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
2 S4 W4 S6 ^$ rUsing In Vivo Biotinylation+ R5 k: K2 S) A! h. U
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem; X8 y* X) V# d1 g
Cells
+ L) d2 h. F' G9 G( T7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional/ e' z& L' }9 ]3 ]2 {
Profiling- j6 L$ o  t3 W" v6 L% g' j
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
7 Q3 e0 ?3 E! `. B& c1. Introduction' h! ?- C0 A5 X2 I
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro/ ]- {( [3 o7 E5 l4 {
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture- u) {! N2 q$ I) ^9 Y6 ?
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of2 r9 M3 q: C$ q0 I1 }
Mouse Embryonic Feeder Layer Cells
) Z8 O, y% }* m: h9 x8 r7 v  h2 m7 x5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
' [8 C9 ~; O3 ^4 `. r0 o0 d$ b6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic
5 m7 @+ b& F9 P" p5 r$ H0 t$ yStem Cells
6 g2 |, q( R! a& }# u9 s& o8 E# [2 u7. Unit 1C.6 Isolation of Human Placental Fibroblasts
1 r3 f8 l, ~& J1 P& _' R8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic6 a% ]4 K1 u* N% o# M6 \  b' i
Stem Cells$ F5 a5 E* B& I0 Q/ s4 u0 k
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of, Z* I. Y* g0 x& j7 n  B
ROCK Inhibitor
- i/ |. `- [# T( m! ^10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
/ \% B2 ?/ A3 m* ^' S0 a11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
) U/ R) n+ f& E. A% j, XStem Cells on Mouse Embryonic Fibroblast Feeder Cells
; ?. ]# x% K% f  K* ^/ v% O12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
2 Z! M4 s* S, Z" `' L4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
1 S# w3 b' V% q1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
& o# K. G! ^$ @/ z* Z9 ?* P/ B6 x2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
# e5 `! \6 P+ m. j0 o9 o* {* ZSuspension and Hanging Drop Cultures* K. n& f5 A! f$ [1 B/ |0 i. P7 E
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
( q. u& d8 w# b0 ?and a Description of the Hematopoietic Blast Colony Forming Assay
; N) v3 V. h+ s$ t3 l4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically7 H) U6 C& J' b' g
Defined Culture Conditions
: C7 [3 C3 W8 G, D$ u+ m$ u5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
, F# [7 n- z- a5. Section E Extraembryonic Lineages
3 W! T4 @9 o  z3 p" Z9 p1. Introduction* H) k% o  U# B0 }" n" _9 d
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation! o4 \3 l- P; P2 L: M. W
into Hepatocyte-Like Cells
( t6 n  ?" y2 q  l& \2 h3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta# Q% h5 G8 |& P& P, N
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
6 e! @3 G. J' Q, \5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
6 ~/ v# U3 p3 @; [/ i6 n6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells  g! q. ^/ U0 R2 o% n% b1 h0 I4 J
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
+ K4 L. t7 v8 X: I4 \6. Section F Mesodermal Lineages' v. i! z- T' f; m
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells! O3 T3 v* O4 i$ I8 |7 ?# I3 @
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture- m; ?9 o6 l  W' |, u1 a% R
with Endoderm in Serum-Free Medium
1 ?$ y9 s* k% S$ e* Z2 h6 a3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells9 g* e/ b( S, u( b, ^2 a/ x; e7 u4 ]
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
1 u+ Y; v$ f  `5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells: {: \, ]4 q9 Y
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation
4 A3 k, e& [; P2 Twith Stromal Layers
- _1 }* r. g; P5 f6 e, H7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
/ P4 z7 e, }9 |4 E* o8 iMurine Hematopoietic Progenitors
3 ?* `$ u6 a4 f1 @8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from' d: }0 o# {3 U. H7 N. R4 s! o
Human Embryonic Stem Cells% b. o/ {7 P7 j' I" i
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells7 M6 T  I. p! m, @+ V# m
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac; T5 _5 D* R( r' `/ u- T' I3 b
Progenitor Cells
/ P) M. T; r% v5 E: n9 |11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
6 d) Y7 `0 K- U7 U1 A& aHanging-Drop and Mass Culture Methods% X) u! K/ H# O+ P4 w
7. Section G Endodermal Lineages! p" n- j/ `3 q( y6 ^$ Z
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells0 C; [' ^6 F2 y5 E' O3 ^
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells# Z! P" h- W- }; V
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
- K) y1 L$ q: H' M( d8. Section H Ectodermal Lineages: T0 W- \4 ^/ a# D) V% B% n7 Q
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons, j  w% N! S9 u% m% z
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by7 m% ~# v& E# c- c6 ^9 ^
Two-Photon Microscopy4 o- O- r( ^; [, o! ]
4. Chapter 2 Somatic Stem Cells: I9 t7 L' l5 C# R# Q" B
1. Section A Hematopoietic Stem Cells& |7 O5 ~6 E$ I6 Y9 ^8 R
1. Introduction
/ M( Y1 ]0 ~/ z# e2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density( L3 a' s8 t3 G% R+ f& f" y
Gradient
/ @2 ], a( e7 B2 R3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
" Y6 N3 o( y5 P, E! ~" @4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
, i  ^* E" c7 I; K5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
: F, L, j$ w# @from Adult Mouse Bone Marrow8 j  L  H  H( p! v4 y0 x/ }
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche( C+ k& Z# N) t; |
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
1 O0 z0 V# j0 N  @: W) rEmbryonic Tissues8 i! @+ l0 q5 ^
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells# h0 t. \" i$ p" m2 s+ m9 Z# T
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
* V+ u- m, m/ Q! C10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
1 z2 ]3 R; V2 i/ c( R( m2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
7 ?, e" [/ R+ ~1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
# [1 O( K0 o' _4 ]& P5 CTissues6 H0 L% v4 y  o7 g, ~
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells' I$ t4 q0 U! @) j
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells( f5 \. i/ `8 D& Q! Q7 I( Z; d
3. Section C Cardiovascular Stem Cells5 s: F* G! z3 L
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
5 C2 k7 g/ S# C" k; O7 s/ P9 o2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
6 Y+ h0 D4 G- l3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells& G0 P2 G; g8 {: [# r; X9 O
4. Section D Neural Stem Cells& i" ?! J; t: i: H$ n
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains, h* |$ T& R0 R+ @* v& ^$ q" j# m
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
3 r% k8 W& @4 J  W; w% _3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and- }3 l% O* L" {' N8 E
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
$ q  u' K  o" T4 D+ X& {Forebrain Neural Stem Cells
1 L. S  m% O3 t4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage
% F/ x0 R. c" U: Q7 rProgression, and Maturation
' q% Q* L- r  }6 W5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
, I0 t: ^& O( h3 X" k# KNeurons from Rodent Brains& v6 J/ `# ?* g, N3 I$ t
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
, ~9 H) @; H1 Q/ z; JTechnique% U! H) c0 u, ~& ~/ l2 F0 e9 `' i
5. Section E Germline Stem Cells7 Z! P% L; R! w" O2 F; {
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila( H  Z" L: E! x- ?8 q
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis& L. ~- I( F. g  L" O6 Y
6. Section F Gut Stem Cells+ Y, i# \+ N; _+ V
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells8 K* a( L. I# S' W8 q
7. Section G Lung Stem Cells' y$ y& t4 F, Q; F/ h
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells( s2 J  T9 x% J
5. Chapter 3 Cancer Stem Cells# x, }/ v  k! i1 P1 r6 i9 I% O
1. Unit 3.1 Colon Cancer Stem Cells
/ r' n& [) s+ x4 v2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model$ Y5 F: L) D& I/ }" g
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells2 T$ h$ E+ I& J* x/ u
6. Chapter 4 Manipulation of Potency( f; D" V$ _* e) Z. h' c4 O
1. Section A iPS Cells( E2 q& f8 N, D' D8 k0 t
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming# Y% y$ g1 a6 v# t( d; N/ M/ C
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells. v% i, G$ U4 O. Q( Y# D
2. Section B Nuclear Transfer
& [/ g4 I  N+ b$ U1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency" K" |4 h9 {3 U) U- q
7. Chapter 5 Genetic Manipulation of Stem Cells' W7 C% W* ~( V( s
1. Section A Lineage Tracers in Stem Cells
( h% w0 o7 F& j1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
4 o3 [/ D" z! ?9 V8 e6 B  d9 B8 i! L2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing! T7 `4 O3 v3 y0 Z
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
) K9 O. x8 {9 T# z4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
" Q  L0 {8 a; t6 R2 d/ K5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
2 Z  l" C, F+ N6 Q+ l) S8 UNeocortical Neural Progenitors
0 f# V2 {  |! H5 U7 H% J7 g2. Section B Homologous Recombination in Stem Cells
$ U9 I" q8 {. |7 e6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by* H3 q) K' o; J5 s6 s0 Q9 g
Homologous Recombination
" w3 ~$ a- W" D1 k8. Appendix 1 Useful Information
3 v: _6 F* g/ p# O1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research$ y. b6 r& C; `
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
9 j+ [/ }1 {' J! |7 g4 E7 d& V0 l9. Appendix 2 Laboratory Equipment
# T/ G3 U! a" a5 ?( f' n/ K3 nStandard Laboratory Equipment4 a/ C6 K, R6 |( D* c
10. Appendix Suppliers
- j' {% a; x- Y. }Selected Suppliers of Reagents and Equipment
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