PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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+ C7 Z5 a" g+ ZCurrent Protocols in Stem Cell Biology 2007年完整版 1451页
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% j* f# T% F8 Z9 ~% J- `( COnline ISBN:9780470151808; J# r& ?' f) x  k
DOI: 10.1002/9780470151808
' a  Q  u2 q! YTable of Contents
7 ]" z( }5 @% _1 x  r+ Q% e1. Foreword
% w. v9 {) p, H: }3 ~) t- D2. Preface6 C! Y5 ^/ o5 x/ o
3. Chapter 1 Embryonic and Extraembryonic Stem Cells! \$ x; K- ^& @' g9 h) k6 [
1. Section A Isolation of Embryonic Stem Cells! o/ p/ z2 b: u1 @$ N7 k
1. Introduction
! c9 k0 G7 n# H/ \( M' s2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells) }9 C  z& j: `3 u' s
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
0 \$ \4 Y/ P; G5 U' i4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells0 m* `2 S  M3 j7 _1 m
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment! n5 B: a8 c4 |/ f
2. Section B Characterization of Embryonic Stem Cells, I2 s. Q% c! u9 F: ?
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells# J  |7 O9 ]3 S. G) m- b6 h0 n
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and/ L" B! J! Z; N! Q2 ]7 U
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform$ |  ?! q7 e( r1 h6 B
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
* k' ?8 U- {' h3 o4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of" M1 e# m$ C! Q9 R; D  G- d/ d1 g
Pluripotency
' a; i+ g: j: x5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
& V( j+ C9 s1 L) s) {1 ^+ |3 W$ TUsing In Vivo Biotinylation1 W3 ^+ d6 E7 F: z8 F4 E- v! u' D
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem" S: X% V# V0 s/ W
Cells, ^. p0 `* N8 U0 U  T' I$ T
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
; h: b5 y# n8 B6 vProfiling" R4 p8 Z& i: y( p) |/ G9 N
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells9 T3 R6 J; b$ W% J3 A. Z' H
1. Introduction
% j5 U; ?! y; g9 n/ y3 D2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
& Q! Y( E  l$ s# x6 |3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
) g& o/ H3 _7 A4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
5 E; L6 E$ ^4 p/ j: `' }6 A/ iMouse Embryonic Feeder Layer Cells+ t- `  U/ \( L* n& o, h
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells  @, ~& J! Y( f; E7 Z! ]5 _4 a
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic9 z$ O" a/ J( l0 D, E8 O* p
Stem Cells
& z: z% r) M! V, Y7. Unit 1C.6 Isolation of Human Placental Fibroblasts9 q$ M3 |8 x# d4 e
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic2 b3 w  E- t5 k! H
Stem Cells
& D) G9 G, \7 e- f4 _: H: H4 W9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of/ s7 A+ [5 e9 _0 q" s
ROCK Inhibitor
- Y4 r; O  O/ Z, t8 c+ N* L10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells/ D9 x2 D8 x* {# M, W9 M; I8 P9 d* |
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent& e1 o8 s$ J8 S; r5 W  z
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
4 i/ v$ K* N& A# {$ ^. o- z12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
! C0 E  O- y. o9 c4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells5 H, c: t) [  A* v5 E& z
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
( h2 q8 o& J0 G3 I2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
# ?' B9 \  t- Y6 T  f$ @/ hSuspension and Hanging Drop Cultures
. }+ U; E7 C! K& b/ c$ |' }3 C3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
2 f$ R$ S' C3 ~" w+ Sand a Description of the Hematopoietic Blast Colony Forming Assay6 ]+ l4 h( w" p6 X/ d; {1 {
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically
. U& u& ?. ^* K8 u+ t- yDefined Culture Conditions
$ l) T5 T# Y; u% u) G& {: D5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
- n7 a7 [' o  A* e) `5. Section E Extraembryonic Lineages
; a) C+ e: g  G& m' S) R3 V1. Introduction) B0 S  |! M- _# O
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
7 f( w7 V6 o  y1 f& F4 B  Finto Hepatocyte-Like Cells
& J" U: N3 \  S2 E4 Z3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta) M" _: m/ l" A: U& ^7 P8 x# |: L
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells' R$ V. B. Q: N( r6 V0 ]9 a1 z+ P
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells0 F- w  R; y* h0 @4 Z
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells( G5 {+ K, n5 A5 Z
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
2 a  h) t& |( s1 V! \/ T/ ]" t6. Section F Mesodermal Lineages
" q/ w% C6 y. {) @1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
4 u# Q2 C: M& }8 m6 d5 [  N1 C2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
7 H% o- Q/ p8 H, m% {6 Ewith Endoderm in Serum-Free Medium6 a" {$ x  [) Z$ m; n+ Q1 u. k* f
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells/ ?: [) L* w. f# h
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
: W: }* N# b( f, ?5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
4 K# l$ G8 R7 e1 S* G6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation& T5 E5 N1 z9 e% x
with Stromal Layers3 M3 s7 j, y. V1 g" b
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
3 H" i; `4 Y5 u: u7 W( e$ UMurine Hematopoietic Progenitors/ i' N) q) P6 o2 n1 O# d
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from! M, r8 W, l4 y4 e) M! V
Human Embryonic Stem Cells# v* b8 M! I7 a' }4 L
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells- }' H# M/ h3 |6 S- i0 n9 [+ a2 ?
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
+ R$ W2 ?( K8 Y9 t* Q$ kProgenitor Cells: Q9 J- ]5 [2 s& [
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the+ [& W. t! x6 O" L
Hanging-Drop and Mass Culture Methods
0 c& b1 f$ \/ O( u! ~( }9 g; {7. Section G Endodermal Lineages
6 G$ V9 B2 u8 \( r8 N1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells1 K/ L+ Z! r* q3 y' Z
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells! Y' \9 `1 u3 |( Y7 l1 ~+ X4 @3 S
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm+ X) Y; H) A" X# I; i+ N
8. Section H Ectodermal Lineages
0 ?2 ]3 r1 f  U- f$ e. y1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons$ w0 J" G# B3 V; O  t
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by
" }: n8 g- b" `# ZTwo-Photon Microscopy- j9 Q/ U* {" m3 J" q( o0 \, h
4. Chapter 2 Somatic Stem Cells% g' P2 a, _4 G8 Q" @4 b
1. Section A Hematopoietic Stem Cells  v- v. e  ~- P; p5 m
1. Introduction1 Y  @9 x% w! {1 D
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density* ~/ A# j% D; A  d5 {& }
Gradient' x; Z$ C7 a0 t- O: L" U
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
9 q) j5 _* M- i3 O* Y! l4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
5 ]5 g- H, i3 }8 i6 _  l1 l$ N7 v5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells7 k  r# n$ ?& ~; @1 N7 o
from Adult Mouse Bone Marrow
% s$ H* i0 {% G8 [. _6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche8 f3 m: b. o* |7 H5 x
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine. {# F2 A0 Q5 z5 [' E
Embryonic Tissues1 H$ Y1 n: Z! p0 B$ N* m8 N
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells" W" V* k/ C8 T/ ]9 A3 m
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
4 G7 S- t& S% q& p10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta) A6 @5 }% q3 {2 Y: @) j
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
. f$ H: |$ K! }! G& O1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human% J% o2 y: `- L7 `+ B
Tissues& ?) h% I$ P4 w! m$ d. e0 ]
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells1 |6 F( p' _) N3 }1 B+ Q1 u& H
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
# F1 L# o# e, ~- ], V3. Section C Cardiovascular Stem Cells# \2 ?6 w3 p! U& t7 O
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood, q  Y4 B4 _) M3 {3 I" y1 x
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium/ G  C" @. \; e/ c. z# P4 ^1 {
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells8 `8 j+ l" T2 o7 f
4. Section D Neural Stem Cells& ]+ C% t) g( C7 O: M- G
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains& h" i7 [" v  R/ t6 U
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells2 {* a& S+ {, P+ ]8 w: x& K
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and& y2 Y+ ?0 R+ v. \" \, s" Y
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal  f9 H) j$ d. ?5 ^
Forebrain Neural Stem Cells
: c; T+ u; [4 g2 w( F4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage4 g$ |* `: {3 w4 U6 \
Progression, and Maturation' y# D& q, ?; W
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic" K9 e- w! e# Q/ t/ g7 |
Neurons from Rodent Brains3 ]( I/ m5 d) J" }9 {
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
8 o; a. X9 v: k% B5 \4 l4 M5 ZTechnique
# e  s0 _# V* i, ]" D9 E% z0 O5 k5. Section E Germline Stem Cells% X# k8 U  r7 T& ~% L& _
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila+ s$ p' ?: r* `
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis! }) S  G4 g$ d
6. Section F Gut Stem Cells9 P, p7 ?5 Y8 U7 I& ^3 e& c5 |
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells) l# p* f9 I! g
7. Section G Lung Stem Cells
5 ~5 y3 \" T4 |# n9 R% Y1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
, s/ ~) T5 }/ n8 F5. Chapter 3 Cancer Stem Cells1 T# J0 [% a8 v3 A* K
1. Unit 3.1 Colon Cancer Stem Cells4 a/ h) @# e/ C
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
1 S0 B: @8 r/ C+ R  [  K! U9 M% }6 x2 V3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells' u8 x  l' G! f8 @; E0 |
6. Chapter 4 Manipulation of Potency+ ?( X6 }1 l; c+ }) x
1. Section A iPS Cells* E/ n+ b& }5 j+ `- t7 j* }+ z
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming+ {$ `# ~* O8 S* I! Y* L+ r8 U& [
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells% e. a( M$ P; `3 ~5 R
2. Section B Nuclear Transfer; r0 s5 k. }# L" q
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency! F2 p5 c3 x4 F' g
7. Chapter 5 Genetic Manipulation of Stem Cells# g' l0 n6 I; A, h" ^
1. Section A Lineage Tracers in Stem Cells
( a" \' o. q; W3 Q1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
# I6 T, [+ R, J/ N3 d2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing7 c" c9 a" ~- G: U- L
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
7 m' j( c" H& S3 x. {" N/ D& E4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
6 d- n( Z. D  h5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian# ]# M& b' T& l( n* u% ]1 _2 [/ d
Neocortical Neural Progenitors2 l/ h; [1 x8 k' [& J1 ^
2. Section B Homologous Recombination in Stem Cells: V$ k8 C  U+ b
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
) _' I# g3 @+ y0 [8 f1 vHomologous Recombination
1 u: f, M; E3 C1 h/ T1 ?8. Appendix 1 Useful Information; w. z* V3 b4 K& Q" {# P" I: h9 q
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research2 W* O4 n& {9 E; o
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells, M! d- h& Y$ Y6 A
9. Appendix 2 Laboratory Equipment  R# P0 G( z" K  ?  P3 i
Standard Laboratory Equipment
% `0 n- }) Y: r; B( w; Q% @: k% `10. Appendix Suppliers
* A( R! M6 W, K8 h1 U5 v4 ]) rSelected Suppliers of Reagents and Equipment4 d) _. O0 p/ q0 \* V5 H

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