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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑
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Contents
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Preface ........................................................................................................v1 i; _7 v" e0 R
Contributors ............................................................................................ xiii) H- {/ K/ w. q# o |
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PART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES
/ @4 v* k# }' L$ u" E6 A1 Transduction Technologies, @7 x. o% s7 Q6 O
David A. Fluri and Martin Fussenegger ........................................... 3; W# V F- ^+ c' ]$ n
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,! N7 z- A9 N. @
and Otto-Wilhelm Merten ........................................................ 232 H) o+ l! V0 I# n! F
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
8 d# X! Z& v) G u( w& m- I+ V1 \! pAlexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93
& @2 v: @ M0 Z! {7 }; m2 `. H4 DNA Fingerprinting and Characterization of Animal Cell Lines W d3 P3 u( O: P# F# t. d! u& C
Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123* y+ V( G* l X% ]: T" s7 R; ~4 l
3 ~- b9 k, J! a3 M5 JPART II. BASIC CULTIVATION TECHNIQUES$ o. h3 O f1 a
5 Microcarrier Cell Culture Technology6 {2 H0 g, g# S+ q0 c+ \3 a
Gerald Blüml ............................................................................... 1497 i: M0 d0 l, R$ i' N) ]
6 Cell Encapsulation
' @4 K0 _4 L; y9 vHolger Hübner ............................................................................ 179 Y9 L. m# g6 h1 Y
7 Tools for High-Throughput Medium and Process Optimization
( \. ~! U* b( {* rMartin Jordan and Nigel Jenkins ................................................. 193
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( p' q1 m' y D- HPART III. CELL CHARACTERIZATION AND ANALYSIS
' g9 e# P, H% v$ O8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205- K; ^, s3 L- |3 `
9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry
; C8 o8 F' c6 y6 y& t3 ?! |Silvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 223) z4 d @, J* }# k
10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
9 j: m9 n8 e& j3 T4 {André A. Neves and Kevin M. Brindle ........................................ 2396 z8 Q: y) ^! h! p" ?- k% }
11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture5 G4 n( P" |( @1 {
Heino Büntemeyer....................................................................... 253. N- S' v8 t/ o4 D" w
12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation
$ ?. X4 C* z0 |8 f9 fDerek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 2694 A' V' F: R. Y
13 Measurement of Apoptosis in Cell Culture, A* C. S9 F" p4 q
Adiba Ishaque and Mohamed Al-Rubeai ..................................... 285
- r: @. `9 L4 F, q14 Metabolic Flux Estimation in Mammalian Cell Cultures
" J; _6 Y/ T% G* y5 KChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 301' g9 t/ p F0 I% i% e. ?- o9 l3 V
PART IV. SPECIAL CULTIVATION TECHNIQUES
/ W- q* r8 V& o15 Disposable Bioreactors for Inoculum Production and Protein Expression( k$ q9 a3 P7 [/ q8 ^
Regine Eibl and Dieter Eibl .......................................................... 321
% Q7 v* P9 ~1 N5 @! a4 y16 Hollow Fiber Cell Culture
: r! C- g% |/ F6 X2 D8 g# yJohn M. Davis .............................................................................. 3378 {0 l$ _$ @1 s I+ N( W6 Q
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors
# U# o" u; c9 T+ N' G( oRalf Pörtner and Oscar B. J. Platas Barradas .............................. 353( J* s& f3 [4 G q8 {( x
18 Configuration of Bioreactors& Y! f% s. a3 o4 t+ a0 R
Dirk E. Martens and Evert Jan van den End ................................ 371
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% W/ b+ y4 C5 ]+ C1 cPART V. DOWNSTREAM TECHNIQUES$ i2 o% h7 R% {6 O7 r1 q
19 Membrane Filtration in Animal Cell Culture& F& l) `, S# h! w# H9 f1 |2 S
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397
! G( K- U' Y9 o1 ]) W20 Chromatographic Techniques in the Downstream Processing. m H q N6 A; m3 c
of (Recombinant) Proteins
1 }' e$ k2 O2 bRuth Freitag ................................................................................. 421
2 S' ^* n2 p4 VPART VI. SPECIAL APPLICATIONS- Z9 K0 R3 y, r q- r
21 Vaccine Production: State of The Art and Future Needs
" K. X2 j+ Q' ~7 Z1 z/ iin Upstream Processing7 w* m* ]$ P, @% w3 a6 j2 Z
Yvonne Genzel and Udo Reichl .................................................. 457- o- Y% l* W7 S$ i/ |" g! _! P
22 Retrovirus Production and Characterization
# R# C" ?5 M$ a' I- TPedro E. Cruz, Marlene Carmo, Teresa Rodrigues,: _5 \ e/ h/ f
and Paula Alves ....................................................................... 475& ?: p7 B0 J: |9 k5 K: Y
23 Insect Cell Cultivation and Generation of Recombinant, I- g5 U2 n- I+ g4 R3 k" o
Baculovirus Particles for Recombinant Protein Production
% P% }! u% D" O4 R+ W8 ~! XSabine Geisse ............................................................................... 4899 n( u- \/ u: }, I1 }" V( u! I
Index ...................................................................................................... 5096 H# `& H. s9 a' U( X' r) x
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发表于 2011-3-31 12:51
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