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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑 & ?5 Q; f2 v: M4 s
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附加上目录，以供需要的人参考。" j7 r2 u9 E( h
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Contents
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n* X; J9 m- l/ H2 r0 e/ TPreface ........................................................................................................v
7 b; z( E: p1 o& R8 C* JContributors ............................................................................................ xiii
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PART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES$ J9 y0 F$ v/ q
1 Transduction Technologies e! t6 d+ D- ^) i- ]: c+ L. \: H
David A. Fluri and Martin Fussenegger ........................................... 3; `3 r z! s, l) T7 ?& a
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,& f Y5 l, n$ b( b
and Otto-Wilhelm Merten ........................................................ 233 f. Z( K2 t5 n- T2 y
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
( n, X! T& C* |% w( R% f0 uAlexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93
9 }% F* E. @' A9 y. q4 DNA Fingerprinting and Characterization of Animal Cell Lines, ^! u7 ?4 s0 |5 A' L6 X
Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123
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}3 v; ? G- B A$ ^PART II. BASIC CULTIVATION TECHNIQUES
, o) J; Z6 n& Q% i" h k) r0 ]$ h5 Microcarrier Cell Culture Technology9 m" R' i |6 P) u6 A9 y
Gerald Blüml ............................................................................... 149$ A) g, p/ H {, M
6 Cell Encapsulation# f5 b4 U9 C8 U: C) H7 j4 i4 W8 h
Holger Hübner ............................................................................ 179
1 E' e2 ?7 b, R) F% n( O7 Tools for High-Throughput Medium and Process Optimization: s6 d. c/ k2 N& k: d! o
Martin Jordan and Nigel Jenkins ................................................. 1933 o0 Z8 t' m: K# _6 G& Z
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PART III. CELL CHARACTERIZATION AND ANALYSIS
. L2 q7 b5 v6 f5 x [8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205
, e1 f2 X, F4 |9 T9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry
6 O& J, J) V9 u# u$ `7 f' [. a: CSilvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 223
/ g6 b% E1 ?$ o" a10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
9 b' Y7 [% Z$ p$ d) ?& K9 dAndré A. Neves and Kevin M. Brindle ........................................ 239
7 G+ a4 }; G0 g$ K- [3 I7 q11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
- l5 P2 I- P U. Q( C. G, k4 }Heino Büntemeyer....................................................................... 2537 v) U5 K. P1 i I3 }+ V
12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation6 x V9 f+ C: B' O# E" Z) |
Derek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269
4 _5 H3 D$ p! I% D$ b13 Measurement of Apoptosis in Cell Culture. h3 z$ U5 v% U. M, a. O
Adiba Ishaque and Mohamed Al-Rubeai ..................................... 285
4 z q3 U+ C% s6 C! O% d14 Metabolic Flux Estimation in Mammalian Cell Cultures
4 [) ^! L8 M$ h- DChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 301) M% r0 e4 |7 c, q
PART IV. SPECIAL CULTIVATION TECHNIQUES
' r. c( p, m( s: C9 M: m0 v. I2 i15 Disposable Bioreactors for Inoculum Production and Protein Expression. w5 J( l/ `, l" @+ `7 {1 o0 F
Regine Eibl and Dieter Eibl .......................................................... 321
& a- @; Z! S( D' n! G) _& P: @7 ~16 Hollow Fiber Cell Culture
- m9 O6 W7 `# m T. w2 X9 OJohn M. Davis .............................................................................. 337/ i& T1 ^: m4 t5 ?
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors* M: \$ |4 y6 }6 t
Ralf Pörtner and Oscar B. J. Platas Barradas .............................. 3536 p$ H. p( ]$ W; c- ^7 ~6 M
18 Configuration of Bioreactors, ?* V' _ j& v* h- P( `
Dirk E. Martens and Evert Jan van den End ................................ 371
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/ K5 E+ s& i6 G1 Z4 T4 U$ F! ]PART V. DOWNSTREAM TECHNIQUES' v/ {) O3 n; w
19 Membrane Filtration in Animal Cell Culture- e. E, i) Q8 |+ j
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397
) F$ J2 p$ a. ]3 o3 i. h20 Chromatographic Techniques in the Downstream Processing) _$ }1 Z) h; b/ N3 X
of (Recombinant) Proteins. J' p. s( |& H# c7 z" I E4 S
Ruth Freitag ................................................................................. 421
& {! P. i: e8 [* h! mPART VI. SPECIAL APPLICATIONS
. o. H. |' N/ \& q) u) D21 Vaccine Production: State of The Art and Future Needs
' A2 V4 F4 ^3 e. ~* e. cin Upstream Processing# x3 h4 k) _! N$ F! a2 k) `1 N' w
Yvonne Genzel and Udo Reichl .................................................. 457
3 g' |6 c8 o- d% d. b22 Retrovirus Production and Characterization9 b' _4 o9 p1 [
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues,
5 ?, K! p9 U8 p" g ?' Tand Paula Alves ....................................................................... 4753 c' J6 l/ g2 o1 v
23 Insect Cell Cultivation and Generation of Recombinant
3 W. T7 h- y4 t, P( hBaculovirus Particles for Recombinant Protein Production% b. e7 h( Z7 @2 ~% `! v; w
Sabine Geisse ............................................................................... 4893 n0 [# |* ^) n- s. x9 x
Index ...................................................................................................... 509
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