Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts # [/ B4 i2 E+ C P3 H Q这篇文章里面用的是混合配方: * b5 U) P9 a5 U4 i2 `Cells were resuspended in transfection media (75% cytosalts [120 mM KCl, 0.15 mM CaCl2, 10 mM K2HPO4; pH 7.6, 5 mM MgCl2] (van den Hoff et al.1992) and 25% Opti-MEM) 4 m% E6 W$ M& \2 F; g * e0 T+ K% v$ ^; O4 T6 b% n' g: K0 i
Hank’s buffer：. p/ E4 F/ N# K' ^# f* [1 t
0 y2 E6 w6 b& \2 j7 @# d; a
137 mM NaCl; 1 h$ B5 d, o& j5 Q! M/ y5 z
5.4 mM KCl; . {3 t3 { e2 ~" |! r* e- X
0.95 mM CaCl2; 7 c. u6 V @3 m" P) i5 K4 D0.41 mM MgSO4; 3 K, t* s% d" u( r/ `! z. C
0.49 mM MgCl2; ) U8 _9 r8 k) C0 z2 ^4.17 mM NaHCO3; : S5 [0 n+ [- m( t
0.34 mM Na2HPO4; " x: ~. j% n! k' ~ l
0.44 mM KH2PO4; 8 M$ g% Y% m2 c20 mM HEPES, pH 7.2 ( z9 z7 \9 s1 W7 k6 K; n
3 I" T5 h J5 m0 H/ G可以直接购买来自HyClone Hanks' Balanced Salt Solutions(HBSS)。" ?: x# Z1 @5 J& C8 m6 M
来自文章---Efficient expression of foreign genes in CHO DHFR(-) cells by electroporation
发表于 2012-5-28 20:28
