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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑 4 P6 b2 M8 U. x9 E7 G! N9 |
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Table of Contents% P K4 c5 T5 v/ W4 G: W7 k7 v
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
: w, h' R9 a- oChapter 2: Bacteriophage and Its Vectors+ b! Y' M' K/ I2 v& {8 n/ x
Chapter 3: Working with Bacteriophage M13 Vectors
- @) n; I1 N/ P( T' nChapter 4: Working with High-Capacity Vectors
- F$ A$ a8 W7 S* @. ]Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
9 k i) l# f- b7 }6 LChapter 6: Preparation and Analysis of Eukaryotic Genomic DNA9 ~( w6 x3 ]6 w- X8 @5 R% }4 w
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells8 y3 Z4 C( _8 w( a+ E& y9 k
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
' W$ f% T0 o! V7 d) D: p+ zChapter 9: Preparation of Radiolabeled DNA and RNA Probes
+ V" g* ~5 K, @( X8 \9 YChapter 10: Working with Synthetic Oligonucleotide Probes
# e6 U( I& h6 y c$ nChapter 11: Preparation of cDNA Libraries and Gene Identification
) e( B. V/ ~# x, {' JChapter 12: DNA Sequencing
3 H7 W* Q$ E9 H; C7 @Chapter 13: Mutagenesis
3 c( q7 I t; K F6 X2 U/ l! LChapter 14: Screening Expression Libraries) n& H3 U# b( M2 o1 A; f7 R
Chapter 15: Expression of Cloned Genes in Escherichia coli
& g! f" T/ G2 _. e$ p Z _8 `Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
3 w+ r! ?6 R' v' ]Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells- \/ D% v8 q- t; L) U) [5 z
Chapter 18: Protein Interaction Technologies4 ?+ ~6 ?2 S1 P: ~& g; e
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